Reducing Tumour Hypoxia via Oral Administration of Oxygen Nanobubbles

Joshua Owen, Conor McEwan, Heather Nesbitt, Phurit Bovornchutichai, Raymond Averre, Mark Borden, Anthony McHale, John F Callan, Eelanor Stride

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Abstract

Hypoxia has been shown to be a key factor inhibiting the successful treatment of solid tumours. Existing strategies for reducing hypoxia, however, have shown limited efficacy and/or adverse side effects. The aim of this study was to investigate the potential for reducing tumour hypoxia using an orally delivered suspension of surfactant-stabilised oxygen nanobubbles. Experiments were carried out in a mouse xenograft tumour model for human pancreatic cancer (BxPc-3 cells in male SCID mice). A single dose of 100 μL of oxygen saturated water, oxygen nanobubbles or argon nanobubbles was administered via gavage. Animals were sacrificed 30 minutes post-treatment (3 per group) and expression of hypoxiainducible-factor-1α (HIF1α) protein measured by real time quantitative polymerase chain reaction and Western blot analysis of the excised tumour tissue. Neither the oxygen saturated water nor argon nanobubbles produced a statistically significant change in HIF1α expression at the transcriptional level. In contrast, a reduction of 75% and 25% in the transcriptional and translational expression of HIF1α respectively (p
Original languageEnglish
Pages (from-to)e0168088
JournalPLoS ONE
Volume11
Issue number12
DOIs
Publication statusPublished - 30 Dec 2016

Keywords

  • Nanobubbles
  • Oxygen
  • Hypoxia
  • HIF-1 alpha

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    Owen, J., McEwan, C., Nesbitt, H., Bovornchutichai, P., Averre, R., Borden, M., McHale, A., Callan, J. F., & Stride, E. (2016). Reducing Tumour Hypoxia via Oral Administration of Oxygen Nanobubbles. PLoS ONE, 11(12), e0168088. https://doi.org/10.1371/journal.pone.0168088