Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K+ channels, expressed on the plasma membrane of pancreatic β-cells, without destroying the β-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.

Original languageEnglish
Title of host publicationPotassium Channels
Subtitle of host publicationMethods and Protocols
EditorsJonathan D. Lippiat
Pages151-164
Number of pages14
DOIs
Publication statusPublished - 1 Dec 2008

Publication series

NameMethods in Molecular Biology
Volume491
ISSN (Print)1064-3745

Keywords

  • ATP-sensitive K channels
  • Bacterial toxins
  • Membrane permeabilisation
  • Open-cell cell-attached configuration
  • Patch-clamp

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  • Cite this

    Tarasov, A. I. (2008). Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration. In J. D. Lippiat (Ed.), Potassium Channels: Methods and Protocols (pp. 151-164). (Methods in Molecular Biology; Vol. 491). https://doi.org/10.1007/978-1-59745-526-8-12