Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

RK Hughes; Z Wu; AR Hornostaj; DS Robinson; D Hardy; SI West; Richard Owusu-Apenten; SA Fairhurst; M Chan; DM Lawson; R Casey

Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

RK Hughes, Z Wu, AR Hornostaj, DS Robinson, D Hardy, SI West, Richard Owusu-Apenten, SA Fairhurst, M Chan, DM Lawson, R Casey

Faculty Of Life & Health Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.

Original language	English
Pages (from-to)	3-6
Journal	Agro Food Industry Hi-Tech
Volume	10
Issue number	3
Publication status	Published (in print/issue) - 1999

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title = "Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.",

abstract = "Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.",

author = "RK Hughes and Z Wu and AR Hornostaj and DS Robinson and D Hardy and SI West and Richard Owusu-Apenten and SA Fairhurst and M Chan and DM Lawson and R Casey",

year = "1999",

language = "English",

volume = "10",

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journal = "Agro Food Industry Hi-Tech",

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T1 - Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

AU - Hughes, RK

AU - Wu, Z

AU - Hornostaj, AR

AU - Robinson, DS

AU - Hardy, D

AU - West, SI

AU - Owusu-Apenten, Richard

AU - Fairhurst, SA

AU - Chan, M

AU - Lawson, DM

AU - Casey, R

PY - 1999

Y1 - 1999

N2 - Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.

AB - Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.

M3 - Article

VL - 10

SP - 3

EP - 6

JO - Agro Food Industry Hi-Tech

JF - Agro Food Industry Hi-Tech

IS - 3

ER -

Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

Abstract

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