Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

RK Hughes, Z Wu, AR Hornostaj, DS Robinson, D Hardy, SI West, Richard Owusu-Apenten, SA Fairhurst, M Chan, DM Lawson, R Casey

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.
LanguageEnglish
Pages3-6
JournalAgro Food Industry Hi-Tech
Volume10
Issue number3
Publication statusPublished - 1999

Fingerprint

Lipoxygenases
Biocatalysts
Peas
Solanum tuberosum
lipoxygenase
Catalysis
peas
Industry
potatoes
industry
Enzymes
Seed
catalytic activity
biocatalysts
pulp and paper industry
reaction mechanisms
Sequence Analysis
Seeds
tubers
chemistry

Cite this

Hughes, RK., Wu, Z., Hornostaj, AR., Robinson, DS., Hardy, D., West, SI., ... Casey, R. (1999). Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. 10(3), 3-6.
Hughes, RK ; Wu, Z ; Hornostaj, AR ; Robinson, DS ; Hardy, D ; West, SI ; Owusu-Apenten, Richard ; Fairhurst, SA ; Chan, M ; Lawson, DM ; Casey, R. / Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. 1999 ; Vol. 10, No. 3. pp. 3-6.
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abstract = "Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.",
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Hughes, RK, Wu, Z, Hornostaj, AR, Robinson, DS, Hardy, D, West, SI, Owusu-Apenten, R, Fairhurst, SA, Chan, M, Lawson, DM & Casey, R 1999, 'Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.', vol. 10, no. 3, pp. 3-6.

Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. / Hughes, RK; Wu, Z; Hornostaj, AR; Robinson, DS; Hardy, D; West, SI; Owusu-Apenten, Richard; Fairhurst, SA; Chan, M; Lawson, DM; Casey, R.

Vol. 10, No. 3, 1999, p. 3-6.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

AU - Hughes, RK

AU - Wu, Z

AU - Hornostaj, AR

AU - Robinson, DS

AU - Hardy, D

AU - West, SI

AU - Owusu-Apenten, Richard

AU - Fairhurst, SA

AU - Chan, M

AU - Lawson, DM

AU - Casey, R

PY - 1999

Y1 - 1999

N2 - Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.

AB - Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.

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Hughes RK, Wu Z, Hornostaj AR, Robinson DS, Hardy D, West SI et al. Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. 1999;10(3):3-6.