Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.
|Journal||Agro Food Industry Hi-Tech|
|Publication status||Published - 1999|
Hughes, RK., Wu, Z., Hornostaj, AR., Robinson, DS., Hardy, D., West, SI., Owusu-Apenten, R., Fairhurst, SA., Chan, M., Lawson, DM., & Casey, R. (1999). Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. Agro Food Industry Hi-Tech, 10(3), 3-6.