Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture.

LO Murphy, YHA Abdel-Wahab, QJ Wang, JA Knezetic, J Permnert, J Larsson, AM Hollingsworth, TE Adrian

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17 Citations (Scopus)

Abstract

Overexpression of autocrine growth factors and their receptors has been reported in many human cancers. The study of autocrine-regulated pathways using in vitro culture systems can be hindered by the presence of fetal bovine serum in culture medium. A human pancreatic cancer cell line (HPAF) was slowly weaned from its dependence on fetal bovine serum and subsequently maintained in serum-free conditions. Growth factor secretion studies showed that production of autocrine growth factors such as transforming growth factor alpha, gastrin-releasing peptide, and insulin-like growth factor I from weaned cells increased three times compared with nonweaned cells (p <0.01). The epidermal growth factor and gastrin-releasing peptide receptor densities were also increased in weaned cells (2 times and 2.5 times, respectively, p <0.05). The proliferation of weaned cells cultured continuously in the same medium was significantly greater than of nonweaned cells (p <0.05). Collectively, these data indicate that weaned pancreatic cancer cells can proliferate in the absence of serum by up-regulating autocrine pathways.
Original languageEnglish
Pages (from-to)293-298
JournalPancreas
Volume22
Issue number3
Publication statusPublished - Apr 2001

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    Murphy, LO., Abdel-Wahab, YHA., Wang, QJ., Knezetic, JA., Permnert, J., Larsson, J., Hollingsworth, AM., & Adrian, TE. (2001). Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. Pancreas, 22(3), 293-298.