Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture.

LO Murphy, YHA Abdel-Wahab, QJ Wang, JA Knezetic, J Permnert, J Larsson, AM Hollingsworth, TE Adrian

Research output: Contribution to journalArticle

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Abstract

Overexpression of autocrine growth factors and their receptors has been reported in many human cancers. The study of autocrine-regulated pathways using in vitro culture systems can be hindered by the presence of fetal bovine serum in culture medium. A human pancreatic cancer cell line (HPAF) was slowly weaned from its dependence on fetal bovine serum and subsequently maintained in serum-free conditions. Growth factor secretion studies showed that production of autocrine growth factors such as transforming growth factor alpha, gastrin-releasing peptide, and insulin-like growth factor I from weaned cells increased three times compared with nonweaned cells (p <0.01). The epidermal growth factor and gastrin-releasing peptide receptor densities were also increased in weaned cells (2 times and 2.5 times, respectively, p <0.05). The proliferation of weaned cells cultured continuously in the same medium was significantly greater than of nonweaned cells (p <0.05). Collectively, these data indicate that weaned pancreatic cancer cells can proliferate in the absence of serum by up-regulating autocrine pathways.
LanguageEnglish
Pages293-298
JournalPancreas
Volume22
Issue number3
Publication statusPublished - Apr 2001

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Pancreatic Neoplasms
Ligands
Growth
Serum
Intercellular Signaling Peptides and Proteins
Bombesin Receptors
Gastrin-Releasing Peptide
Transforming Growth Factor alpha
Growth Factor Receptors
Insulin-Like Growth Factor I
Epidermal Growth Factor
Culture Media
Cultured Cells
Cell Proliferation
Cell Line
Neoplasms

Cite this

Murphy, LO., Abdel-Wahab, YHA., Wang, QJ., Knezetic, JA., Permnert, J., Larsson, J., ... Adrian, TE. (2001). Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. Pancreas, 22(3), 293-298.
Murphy, LO ; Abdel-Wahab, YHA ; Wang, QJ ; Knezetic, JA ; Permnert, J ; Larsson, J ; Hollingsworth, AM ; Adrian, TE. / Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. In: Pancreas. 2001 ; Vol. 22, No. 3. pp. 293-298.
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abstract = "Overexpression of autocrine growth factors and their receptors has been reported in many human cancers. The study of autocrine-regulated pathways using in vitro culture systems can be hindered by the presence of fetal bovine serum in culture medium. A human pancreatic cancer cell line (HPAF) was slowly weaned from its dependence on fetal bovine serum and subsequently maintained in serum-free conditions. Growth factor secretion studies showed that production of autocrine growth factors such as transforming growth factor alpha, gastrin-releasing peptide, and insulin-like growth factor I from weaned cells increased three times compared with nonweaned cells (p <0.01). The epidermal growth factor and gastrin-releasing peptide receptor densities were also increased in weaned cells (2 times and 2.5 times, respectively, p <0.05). The proliferation of weaned cells cultured continuously in the same medium was significantly greater than of nonweaned cells (p <0.05). Collectively, these data indicate that weaned pancreatic cancer cells can proliferate in the absence of serum by up-regulating autocrine pathways.",
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Murphy, LO, Abdel-Wahab, YHA, Wang, QJ, Knezetic, JA, Permnert, J, Larsson, J, Hollingsworth, AM & Adrian, TE 2001, 'Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture.', Pancreas, vol. 22, no. 3, pp. 293-298.

Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. / Murphy, LO; Abdel-Wahab, YHA; Wang, QJ; Knezetic, JA; Permnert, J; Larsson, J; Hollingsworth, AM; Adrian, TE.

In: Pancreas, Vol. 22, No. 3, 04.2001, p. 293-298.

Research output: Contribution to journalArticle

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T1 - Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture.

AU - Murphy, LO

AU - Abdel-Wahab, YHA

AU - Wang, QJ

AU - Knezetic, JA

AU - Permnert, J

AU - Larsson, J

AU - Hollingsworth, AM

AU - Adrian, TE

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N2 - Overexpression of autocrine growth factors and their receptors has been reported in many human cancers. The study of autocrine-regulated pathways using in vitro culture systems can be hindered by the presence of fetal bovine serum in culture medium. A human pancreatic cancer cell line (HPAF) was slowly weaned from its dependence on fetal bovine serum and subsequently maintained in serum-free conditions. Growth factor secretion studies showed that production of autocrine growth factors such as transforming growth factor alpha, gastrin-releasing peptide, and insulin-like growth factor I from weaned cells increased three times compared with nonweaned cells (p <0.01). The epidermal growth factor and gastrin-releasing peptide receptor densities were also increased in weaned cells (2 times and 2.5 times, respectively, p <0.05). The proliferation of weaned cells cultured continuously in the same medium was significantly greater than of nonweaned cells (p <0.05). Collectively, these data indicate that weaned pancreatic cancer cells can proliferate in the absence of serum by up-regulating autocrine pathways.

AB - Overexpression of autocrine growth factors and their receptors has been reported in many human cancers. The study of autocrine-regulated pathways using in vitro culture systems can be hindered by the presence of fetal bovine serum in culture medium. A human pancreatic cancer cell line (HPAF) was slowly weaned from its dependence on fetal bovine serum and subsequently maintained in serum-free conditions. Growth factor secretion studies showed that production of autocrine growth factors such as transforming growth factor alpha, gastrin-releasing peptide, and insulin-like growth factor I from weaned cells increased three times compared with nonweaned cells (p <0.01). The epidermal growth factor and gastrin-releasing peptide receptor densities were also increased in weaned cells (2 times and 2.5 times, respectively, p <0.05). The proliferation of weaned cells cultured continuously in the same medium was significantly greater than of nonweaned cells (p <0.05). Collectively, these data indicate that weaned pancreatic cancer cells can proliferate in the absence of serum by up-regulating autocrine pathways.

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