Rapid loss of estrogenicity of steroid estrogens by UVA photolysis and photocatalysis over an immobilised titanium dioxide catalyst

Heather Coleman, E.J. Routledge, J.P. Sumpter, B.R. Eggins, J.A. Byrne

Research output: Contribution to journalArticle

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Abstract

The presence of low levels of natural and synthetic steroid estrogens in the aquatic environment, and their biological effects on aquatic organisms, are presently issues of concern. In this study, we investigated the temporal removal of estrogenic activity of several potent and environmentally relevant steroid estrogens by photocatalysis over an immobilised titanium dioxide (TiO2) catalyst. We used a recombinant yeast assay to measure estrogenic activity, which provided detection limits within the reactor of 53 ng/l for 17β-estradiol and 17α-ethinylestradiol, and 100 ng/l for estrone. Pseudo-first-order kinetic data showed that photocatalysis over titanium dioxide was equally effective at removing the estrogenic activity of all three steroid substrates in aqueous solutions (initial concentrations of 10 μg/l) with a 50% reduction in estrogenicity within 10 min. In control experiments without TiO2 catalyst, the rate of UVA photolysis of the steroid substrates varied, but was most effective with 17α-ethinylestradiol followed by estrone, and was least effective with 17β-estradiol (0.42, 0.2 and
LanguageEnglish
Pages3233-3240
JournalWater Research
Volume38
Issue number14-15
Early online date22 Jun 2004
DOIs
Publication statusE-pub ahead of print - 22 Jun 2004

Fingerprint

Photocatalysis
Photolysis
steroid
photolysis
Titanium dioxide
catalyst
Aquatic organisms
Catalysts
Substrates
Yeast
Assays
substrate
Kinetics
aquatic organism
aquatic environment
yeast
aqueous solution
Experiments
assay
kinetics

Keywords

  • Titanium dioxide
  • Estrogens
  • Photocatalysis
  • UVA photolysis
  • Recombinant yeast screen

Cite this

Coleman, Heather ; Routledge, E.J. ; Sumpter, J.P. ; Eggins, B.R. ; Byrne, J.A. / Rapid loss of estrogenicity of steroid estrogens by UVA photolysis and photocatalysis over an immobilised titanium dioxide catalyst. In: Water Research. 2004 ; Vol. 38, No. 14-15. pp. 3233-3240.
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Rapid loss of estrogenicity of steroid estrogens by UVA photolysis and photocatalysis over an immobilised titanium dioxide catalyst. / Coleman, Heather; Routledge, E.J.; Sumpter, J.P.; Eggins, B.R.; Byrne, J.A.

In: Water Research, Vol. 38, No. 14-15, 22.06.2004, p. 3233-3240.

Research output: Contribution to journalArticle

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T1 - Rapid loss of estrogenicity of steroid estrogens by UVA photolysis and photocatalysis over an immobilised titanium dioxide catalyst

AU - Coleman, Heather

AU - Routledge, E.J.

AU - Sumpter, J.P.

AU - Eggins, B.R.

AU - Byrne, J.A.

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AB - The presence of low levels of natural and synthetic steroid estrogens in the aquatic environment, and their biological effects on aquatic organisms, are presently issues of concern. In this study, we investigated the temporal removal of estrogenic activity of several potent and environmentally relevant steroid estrogens by photocatalysis over an immobilised titanium dioxide (TiO2) catalyst. We used a recombinant yeast assay to measure estrogenic activity, which provided detection limits within the reactor of 53 ng/l for 17β-estradiol and 17α-ethinylestradiol, and 100 ng/l for estrone. Pseudo-first-order kinetic data showed that photocatalysis over titanium dioxide was equally effective at removing the estrogenic activity of all three steroid substrates in aqueous solutions (initial concentrations of 10 μg/l) with a 50% reduction in estrogenicity within 10 min. In control experiments without TiO2 catalyst, the rate of UVA photolysis of the steroid substrates varied, but was most effective with 17α-ethinylestradiol followed by estrone, and was least effective with 17β-estradiol (0.42, 0.2 and

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