Ranakinin: A Novel NK1 Tachykinin Receptor Agonist Isolated with Neurokinin B from the Brain of the Frog Rana ridibunda

Finbarr O'Harte, Elizabeth Burcher, Sandor Lovas, D. David Smith, Hubert Vaudry, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

An extract of the whole brain of the frog Rana ridibunda contained high concentrations of substance P‐like immunoreactivity, measured with an antiserum directed against the COOH‐terminal region of mammalian substance p and neurokinin b‐like immurtoreactivity, measured with an antiserum directed against the NH2‐terminus of neurokinin B. The primary structure of the substance p‐related peptide (ranakinin) was established as: Lys‐Pro‐Asn‐Pro‐Glu‐Arg‐Phe‐Tyr‐Gly‐Leu‐Met‐NH2. Mammalian substance P was not present in the extract. The primary structure of the neurokinin b‐related peptide was established as: Asp‐Met‐His‐Asp‐Phe‐Phe‐Val‐Gly‐Leu‐Met‐NH2. This amino acid sequence is the same as that of mammalian neurokinin B. Ranakinin was equipotent with substance p and [Sar9,‐Met(O2)11]substance p in inhibiting the binding of 125I‐Bolton‐Hunter‐[Sar9, Met(O2)11]substance p, a selective radio‐ligand for the NK1 receptor, to binding sites in rat subman‐dibular gland membranes (IC50 1.6 ± 0.3 nM; n = 5). It is concluded that ranakinin is a preferred agonist for the mammalian NK1 tachykinin receptor subtype.

Original languageEnglish
Pages (from-to)2086-2091
Number of pages6
JournalJournal of Neurochemistry
Volume57
Issue number6
DOIs
Publication statusPublished - Dec 1991

Keywords

  • O'Harte F. et al. Ranakinin: A novel NK1 tachykinin receptor agonist isolated with neurokinin b from the brain of the frog Rana ridibunda.
  • Tachykinin—Substance p—Neurokinin b—Frog brain

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