TY - JOUR
T1 - Ranakinin
T2 - A Novel NK1 Tachykinin Receptor Agonist Isolated with Neurokinin B from the Brain of the Frog Rana ridibunda
AU - O'Harte, Finbarr
AU - Burcher, Elizabeth
AU - Lovas, Sandor
AU - Smith, D. David
AU - Vaudry, Hubert
AU - Conlon, J. Michael
PY - 1991/12
Y1 - 1991/12
N2 - An extract of the whole brain of the frog Rana ridibunda contained high concentrations of substance P‐like immunoreactivity, measured with an antiserum directed against the COOH‐terminal region of mammalian substance p and neurokinin b‐like immurtoreactivity, measured with an antiserum directed against the NH2‐terminus of neurokinin B. The primary structure of the substance p‐related peptide (ranakinin) was established as: Lys‐Pro‐Asn‐Pro‐Glu‐Arg‐Phe‐Tyr‐Gly‐Leu‐Met‐NH2. Mammalian substance P was not present in the extract. The primary structure of the neurokinin b‐related peptide was established as: Asp‐Met‐His‐Asp‐Phe‐Phe‐Val‐Gly‐Leu‐Met‐NH2. This amino acid sequence is the same as that of mammalian neurokinin B. Ranakinin was equipotent with substance p and [Sar9,‐Met(O2)11]substance p in inhibiting the binding of 125I‐Bolton‐Hunter‐[Sar9, Met(O2)11]substance p, a selective radio‐ligand for the NK1 receptor, to binding sites in rat subman‐dibular gland membranes (IC50 1.6 ± 0.3 nM; n = 5). It is concluded that ranakinin is a preferred agonist for the mammalian NK1 tachykinin receptor subtype.
AB - An extract of the whole brain of the frog Rana ridibunda contained high concentrations of substance P‐like immunoreactivity, measured with an antiserum directed against the COOH‐terminal region of mammalian substance p and neurokinin b‐like immurtoreactivity, measured with an antiserum directed against the NH2‐terminus of neurokinin B. The primary structure of the substance p‐related peptide (ranakinin) was established as: Lys‐Pro‐Asn‐Pro‐Glu‐Arg‐Phe‐Tyr‐Gly‐Leu‐Met‐NH2. Mammalian substance P was not present in the extract. The primary structure of the neurokinin b‐related peptide was established as: Asp‐Met‐His‐Asp‐Phe‐Phe‐Val‐Gly‐Leu‐Met‐NH2. This amino acid sequence is the same as that of mammalian neurokinin B. Ranakinin was equipotent with substance p and [Sar9,‐Met(O2)11]substance p in inhibiting the binding of 125I‐Bolton‐Hunter‐[Sar9, Met(O2)11]substance p, a selective radio‐ligand for the NK1 receptor, to binding sites in rat subman‐dibular gland membranes (IC50 1.6 ± 0.3 nM; n = 5). It is concluded that ranakinin is a preferred agonist for the mammalian NK1 tachykinin receptor subtype.
KW - O'Harte F. et al. Ranakinin: A novel NK1 tachykinin receptor agonist isolated with neurokinin b from the brain of the frog Rana ridibunda.
KW - Tachykinin—Substance p—Neurokinin b—Frog brain
UR - http://www.scopus.com/inward/record.url?scp=0025718773&partnerID=8YFLogxK
U2 - 10.1111/j.1471-4159.1991.tb06426.x
DO - 10.1111/j.1471-4159.1991.tb06426.x
M3 - Article
C2 - 1658233
AN - SCOPUS:0025718773
SN - 0022-3042
VL - 57
SP - 2086
EP - 2091
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 6
ER -