Raman spectroscopy of primary bovine aortic endothelial cells: a comparison of single cell and cell cluster analysis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

There are many techniques that allow in vitro interactions among cells and their environment to be monitored, including molecular, biochemical and immunochemicaltechniques. Traditional techniques for the analysis of cells often require fixation or lysis from substrates; however, use of such destructive methods is not feasible where the expanded cell cultures are required to be used for clinical implantation. Several studies have previously highlighted the potential of Raman spectroscopy to provide useful information on key biochemical markers within cells. As such, we highlight thecapability of Raman spectroscopy with different laser spot sizes for use as a non-invasive, rapid, and specific method to perform in situ analysis of primary bovine aortic endothelial cells (BAECs). Raman spectra were collected from both individual live cells cultured on fused silica substrates and on clusters of live cells placed on fused silica substrates, measured at 532 and 785 nm. The results obtained show notable spectral differences in DNA/RNA region indicative of the relative cytoplasm and nucleus contributions. Raman spectra of cell clusters show slight variations in the intensity of the phenylalanine peak (1004 cm-1) indicating variations in protein contribution. These spectra also highlight contributions from other cellular components such as, proteins, lipids, nucleic acids and carbohydrates, respectively.
LanguageEnglish
Pages1923-1930
JournalJournal of Materials Science: Materials in Medicine
Volume22
DOIs
Publication statusPublished - 2011

Fingerprint

Raman Spectrum Analysis
Endothelial cells
Cluster analysis
Cluster Analysis
Raman spectroscopy
Endothelial Cells
Fused silica
Raman scattering
Substrates
Silicon Dioxide
Proteins
Nucleic acids
Carbohydrates
RNA
Phenylalanine
Cell culture
Nucleic Acids
Lipids
DNA
Cells

Cite this

@article{d99fa2bbf94a472ea6c9181b6a340114,
title = "Raman spectroscopy of primary bovine aortic endothelial cells: a comparison of single cell and cell cluster analysis",
abstract = "There are many techniques that allow in vitro interactions among cells and their environment to be monitored, including molecular, biochemical and immunochemicaltechniques. Traditional techniques for the analysis of cells often require fixation or lysis from substrates; however, use of such destructive methods is not feasible where the expanded cell cultures are required to be used for clinical implantation. Several studies have previously highlighted the potential of Raman spectroscopy to provide useful information on key biochemical markers within cells. As such, we highlight thecapability of Raman spectroscopy with different laser spot sizes for use as a non-invasive, rapid, and specific method to perform in situ analysis of primary bovine aortic endothelial cells (BAECs). Raman spectra were collected from both individual live cells cultured on fused silica substrates and on clusters of live cells placed on fused silica substrates, measured at 532 and 785 nm. The results obtained show notable spectral differences in DNA/RNA region indicative of the relative cytoplasm and nucleus contributions. Raman spectra of cell clusters show slight variations in the intensity of the phenylalanine peak (1004 cm-1) indicating variations in protein contribution. These spectra also highlight contributions from other cellular components such as, proteins, lipids, nucleic acids and carbohydrates, respectively.",
author = "L McManus and A Boyd and GA Burke and BJ Meenan",
year = "2011",
doi = "10.1007/s10856-011-4383-7",
language = "English",
volume = "22",
pages = "1923--1930",
journal = "Journal of Materials Science: Materials in Medicine",
issn = "0957-4530",

}

TY - JOUR

T1 - Raman spectroscopy of primary bovine aortic endothelial cells: a comparison of single cell and cell cluster analysis

AU - McManus, L

AU - Boyd, A

AU - Burke, GA

AU - Meenan, BJ

PY - 2011

Y1 - 2011

N2 - There are many techniques that allow in vitro interactions among cells and their environment to be monitored, including molecular, biochemical and immunochemicaltechniques. Traditional techniques for the analysis of cells often require fixation or lysis from substrates; however, use of such destructive methods is not feasible where the expanded cell cultures are required to be used for clinical implantation. Several studies have previously highlighted the potential of Raman spectroscopy to provide useful information on key biochemical markers within cells. As such, we highlight thecapability of Raman spectroscopy with different laser spot sizes for use as a non-invasive, rapid, and specific method to perform in situ analysis of primary bovine aortic endothelial cells (BAECs). Raman spectra were collected from both individual live cells cultured on fused silica substrates and on clusters of live cells placed on fused silica substrates, measured at 532 and 785 nm. The results obtained show notable spectral differences in DNA/RNA region indicative of the relative cytoplasm and nucleus contributions. Raman spectra of cell clusters show slight variations in the intensity of the phenylalanine peak (1004 cm-1) indicating variations in protein contribution. These spectra also highlight contributions from other cellular components such as, proteins, lipids, nucleic acids and carbohydrates, respectively.

AB - There are many techniques that allow in vitro interactions among cells and their environment to be monitored, including molecular, biochemical and immunochemicaltechniques. Traditional techniques for the analysis of cells often require fixation or lysis from substrates; however, use of such destructive methods is not feasible where the expanded cell cultures are required to be used for clinical implantation. Several studies have previously highlighted the potential of Raman spectroscopy to provide useful information on key biochemical markers within cells. As such, we highlight thecapability of Raman spectroscopy with different laser spot sizes for use as a non-invasive, rapid, and specific method to perform in situ analysis of primary bovine aortic endothelial cells (BAECs). Raman spectra were collected from both individual live cells cultured on fused silica substrates and on clusters of live cells placed on fused silica substrates, measured at 532 and 785 nm. The results obtained show notable spectral differences in DNA/RNA region indicative of the relative cytoplasm and nucleus contributions. Raman spectra of cell clusters show slight variations in the intensity of the phenylalanine peak (1004 cm-1) indicating variations in protein contribution. These spectra also highlight contributions from other cellular components such as, proteins, lipids, nucleic acids and carbohydrates, respectively.

U2 - 10.1007/s10856-011-4383-7

DO - 10.1007/s10856-011-4383-7

M3 - Article

VL - 22

SP - 1923

EP - 1930

JO - Journal of Materials Science: Materials in Medicine

T2 - Journal of Materials Science: Materials in Medicine

JF - Journal of Materials Science: Materials in Medicine

SN - 0957-4530

ER -