Radioadaptation in Indian muntjac fibroblast cells induced by low intensity laser irradiation

KM Joyce, Stephen Downes, BM Hannigan

    Research output: Contribution to journalArticle

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    Abstract

    Earlier reports have indicated that an adaptive, protective response to ionizing radiation is inducible by pre-treatment with low intensity laser irradiation (LILI). We have investigated the potential of LILI to induce an adaptive response against the damaging effects of ionizing radiation in Indian muntjac fibroblasts. LILI at 660, but not 820 nm, at 11.5 and 23.0 J/cm(2), induced an apparent adaptive response in the form of a reduction in the frequency of radiation-induced chromosome aberrations, but not in cell survival. There was also a trend towards a reduction in the level of single-stranded and double-stranded DNA breaks induced by ionizing radiation when cells were preconditioned with LILI. However, this did not contribute to the reduced chromosome aberration frequency. Further analysis revealed that the reduced aberration frequency was caused by a laser-induced extension of G2. delay. The adaptive response was therefore the result of cell cycle modulation by LILI, at a wavelength where there is no known DNA damaging effect to induce the checkpoint mechanisms that are normally responsible for altering cell cycle progression. (C) 1999 Elsevier Science B.V. All rights reserved.
    LanguageEnglish
    Pages35-42
    JournalMUTATION RESEARCH-DNA REPAIR
    Volume435
    Issue number1
    Publication statusPublished - Sep 1999

    Fingerprint

    Muntjacs
    Lasers
    Fibroblasts
    Ionizing Radiation
    Chromosome Aberrations
    Cell Cycle
    Double-Stranded DNA Breaks
    Cell Survival
    Radiation
    DNA

    Cite this

    Joyce, KM ; Downes, Stephen ; Hannigan, BM. / Radioadaptation in Indian muntjac fibroblast cells induced by low intensity laser irradiation. In: MUTATION RESEARCH-DNA REPAIR. 1999 ; Vol. 435, No. 1. pp. 35-42.
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    abstract = "Earlier reports have indicated that an adaptive, protective response to ionizing radiation is inducible by pre-treatment with low intensity laser irradiation (LILI). We have investigated the potential of LILI to induce an adaptive response against the damaging effects of ionizing radiation in Indian muntjac fibroblasts. LILI at 660, but not 820 nm, at 11.5 and 23.0 J/cm(2), induced an apparent adaptive response in the form of a reduction in the frequency of radiation-induced chromosome aberrations, but not in cell survival. There was also a trend towards a reduction in the level of single-stranded and double-stranded DNA breaks induced by ionizing radiation when cells were preconditioned with LILI. However, this did not contribute to the reduced chromosome aberration frequency. Further analysis revealed that the reduced aberration frequency was caused by a laser-induced extension of G2. delay. The adaptive response was therefore the result of cell cycle modulation by LILI, at a wavelength where there is no known DNA damaging effect to induce the checkpoint mechanisms that are normally responsible for altering cell cycle progression. (C) 1999 Elsevier Science B.V. All rights reserved.",
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    Radioadaptation in Indian muntjac fibroblast cells induced by low intensity laser irradiation. / Joyce, KM; Downes, Stephen; Hannigan, BM.

    In: MUTATION RESEARCH-DNA REPAIR, Vol. 435, No. 1, 09.1999, p. 35-42.

    Research output: Contribution to journalArticle

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    AB - Earlier reports have indicated that an adaptive, protective response to ionizing radiation is inducible by pre-treatment with low intensity laser irradiation (LILI). We have investigated the potential of LILI to induce an adaptive response against the damaging effects of ionizing radiation in Indian muntjac fibroblasts. LILI at 660, but not 820 nm, at 11.5 and 23.0 J/cm(2), induced an apparent adaptive response in the form of a reduction in the frequency of radiation-induced chromosome aberrations, but not in cell survival. There was also a trend towards a reduction in the level of single-stranded and double-stranded DNA breaks induced by ionizing radiation when cells were preconditioned with LILI. However, this did not contribute to the reduced chromosome aberration frequency. Further analysis revealed that the reduced aberration frequency was caused by a laser-induced extension of G2. delay. The adaptive response was therefore the result of cell cycle modulation by LILI, at a wavelength where there is no known DNA damaging effect to induce the checkpoint mechanisms that are normally responsible for altering cell cycle progression. (C) 1999 Elsevier Science B.V. All rights reserved.

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