Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site

Reinhard Kage; Finbarr O'Harte; Lars Thim; J. Michael Conlon

doi:10.1016/0167-0115(91)90213-Z

Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site

Reinhard Kage
, Finbarr O'Harte
, Lars Thim
, J. Michael Conlon

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val- Asp-Glu-Glu-Phe-Gln-Ser-Pro¹⁰-Phe-Gly-Ser-Arg-Ser-Arg-Gly-Tyr-Phe-Leu²⁰-Phe- Arg-Pro-Arg-Asn.NH₂. In rabbit neuromedin U, the Arg¹⁶-Arg¹⁷ dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.

Original language	English
Pages (from-to)	191-198
Number of pages	8
Journal	Regulatory Peptides
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/0167-0115(91)90213-Z
Publication status	Published (in print/issue) - 25 Apr 1991

Funding

This work was supported in part by the Stiftung Volkswagenwerk.

Keywords

HPLC
Neuromedin U
Primary structure
Rabbit intestine
Radioimmunoassay

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10.1016/0167-0115(91)90213-Z

Cite this

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title = "Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site",

abstract = "The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val- Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg-Gly-Tyr-Phe-Leu20-Phe- Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.",

keywords = "HPLC, Neuromedin U, Primary structure, Rabbit intestine, Radioimmunoassay",

author = "Reinhard Kage and Finbarr O'Harte and Lars Thim and Conlon, \{J. Michael\}",

year = "1991",

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doi = "10.1016/0167-0115(91)90213-Z",

language = "English",

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TY - JOUR

T1 - Rabbit neuromedin U-25

T2 - lack of conservation of a posttranslational processing site

AU - Kage, Reinhard

AU - O'Harte, Finbarr

AU - Thim, Lars

AU - Conlon, J. Michael

PY - 1991/4/25

Y1 - 1991/4/25

N2 - The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val- Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg-Gly-Tyr-Phe-Leu20-Phe- Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.

AB - The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val- Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg-Gly-Tyr-Phe-Leu20-Phe- Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.

KW - HPLC

KW - Neuromedin U

KW - Primary structure

KW - Rabbit intestine

KW - Radioimmunoassay

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U2 - 10.1016/0167-0115(91)90213-Z

DO - 10.1016/0167-0115(91)90213-Z

M3 - Article

C2 - 1882085

AN - SCOPUS:0025905976

SN - 0167-0115

VL - 33

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EP - 198

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 2

ER -

Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site

Abstract

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Keywords

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