Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site

Reinhard Kage, Finbarr O'Harte, Lars Thim, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val- Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg-Gly-Tyr-Phe-Leu20-Phe- Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalRegulatory Peptides
Volume33
Issue number2
DOIs
Publication statusPublished - 25 Apr 1991

Keywords

  • HPLC
  • Neuromedin U
  • Primary structure
  • Rabbit intestine
  • Radioimmunoassay

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