Quantitative 3D OPT and LSFM datasets of pancreata from mice with streptozotocin-induced diabetes

Max Hahn, Christoffer Nord, Pim P. van Krieken, Per Olof Berggren, Erwin Ilegems, Abbas Cheddad, Ulf Ahlgren

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Abstract

Mouse models for streptozotocin (STZ) induced diabetes probably represent the most widely used systems for preclinical diabetes research, owing to the compound’s toxic effect on pancreatic β-cells. However, a comprehensive view of pancreatic β-cell mass distribution subject to STZ administration is lacking. Previous assessments have largely relied on the extrapolation of stereological sections, which provide limited 3D-spatial and quantitative information. This data descriptor presents multiple ex vivo tomographic optical image datasets of the full β-cell mass distribution in mice subject to single high and multiple low doses of STZ administration, and in glycaemia recovered mice. The data further include information about structural features, such as individual islet β-cell volumes, spatial coordinates, and shape as well as signal intensities for both insulin and GLUT2. Together, they provide the most comprehensive anatomical record of the effects of STZ administration on the islet of Langerhans in mice. As such, this data descriptor may serve as reference material to facilitate the planning, use and (re)interpretation of this widely used disease model.

Original languageEnglish
Article number558
Pages (from-to)1-8
Number of pages8
JournalScientific Data
Volume9
Issue number1
Early online date10 Sep 2022
DOIs
Publication statusPublished online - 10 Sep 2022

Bibliographical note

Funding Information:
This project was funded by the Swedish Research Council, the Kempe foundations, Umeå University, Lenanders stiftelse, The strategic Research program in Diabetes at Karolinska Institutet, the Novo Nordisk Foundation, the Swedish Diabetes Association, the Family Knut and Alice Wallenberg Foundation, Diabetes Research and Wellness Foundation, the Stichting af Jochnick Foundation, the Family Erling-Persson Foundation, Berth von Kantzow’s Foundation, the Skandia Insurance Company, Ltd., ERC-2018-AdG 834860 EYELETS, the European Union’s Seventh Framework Program under grant agreements nos. 289932 and 613879.

Publisher Copyright:
© 2022, The Author(s).

Keywords

  • Animals
  • Blood Glucose/analysis
  • Diabetes Mellitus, Experimental
  • Insulin/analysis
  • Islets of Langerhans
  • Mice
  • Streptozocin/analysis

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