Quantitation and Characterization of Peptides from the C‐Terminal Flanking Region of Rat and Bovine Preprotachykinins

Gerard P. McGregor, Reinhard Kage, Lars Thim, J. Michael Conlon

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8 Citations (Scopus)


Abstract: Sequence analysis of cDNAs has shown that the biosynthetic precursors of substance P (α‐,β‐, and γ‐prepro‐tachykinins) contain a common amino acid sequence in the C‐terminal flanking region that has not been conserved between species. Antisera have been raised against the synthetic peptide Tyr‐Glu‐Arg‐Ser‐Ala‐Met‐Gln‐Asn‐Tyr‐Glu, which represents rat β‐preprotachykinin‐(117‐126)‐peptide, and used in radioimmunoassays. Antiserum R50 reacted strongly with C‐flanking peptides in extracts of rat and bovine tissues whereas antiserum GP‐4 reacted only with the rat peptides. The primary structure of the predominant molecular form of prepro tachykinin C‐flanking peptide in an extract of bovine corpus striatum was established as: Ala‐Leu‐Asn‐Ser‐Val5‐Ala‐Tyr‐Glu‐Arg‐Ser10‐Val‐Met‐Gln‐Asp‐Tyr15‐GIu. This sequence represents β‐preprotachykinin‐(111‐126)‐peptide which is equivalent to γ‐preprotachykinin‐(96‐111)‐peptide. A C‐flanking peptide with similar chromatographic properties was identified in extracts of rat brain and gut together with a second immunoreactive component that may represent a fragment or a posttranslationally modified variant. A peptide corresponding to the 37‐amino‐acid residue C‐flanking peptide derived from α‐preprotachykinin was not detected in the extracts as expected from the known low abundance of SaL‐preprotachykinin mRNA in rat brain and gut.

Original languageEnglish
Pages (from-to)1871-1877
Number of pages7
JournalJournal of Neurochemistry
Issue number6
Publication statusPublished (in print/issue) - Dec 1989


  • Bovine corpus striatum
  • C‐flanking peptide
  • Preprotachykinin
  • Rat gastrointestinal tract
  • Substance P


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