Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma

J. Michael Conlon; Kenneth R. Olson

doi:10.1016/0014-5793(93)81684-R

Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma

J. Michael Conlon, Kenneth R. Olson

School of Biomedical Sciences

University of Notre Dame

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe⁶→Trp and Phe⁹→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

Original language	English
Pages (from-to)	75-78
Number of pages	4
Journal	FEBS Letters
Volume	334
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(93)81684-R
Publication status	Published (in print/issue) - 8 Nov 1993

Funding

Acknowledgements: This work was supported by a National Science Foundation Grants IBN 9105247 and IBN 9117387.

Funders	Funder number
National Science Foundation	IBN 9117387, IBN 9105247

Keywords

Blood pressure
Bradykinin
Kallidin
Kallikrein
Kininogen
Trout

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10.1016/0014-5793(93)81684-R

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title = "Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma",

abstract = "Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6→Trp and Phe9→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.",

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T1 - Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma

AU - Conlon, J. Michael

AU - Olson, Kenneth R.

PY - 1993/11/8

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N2 - Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6→Trp and Phe9→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

AB - Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6→Trp and Phe9→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

KW - Blood pressure

KW - Bradykinin

KW - Kallidin

KW - Kallikrein

KW - Kininogen

KW - Trout

UR - https://www.scopus.com/pages/publications/0027452522

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DO - 10.1016/0014-5793(93)81684-R

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AN - SCOPUS:0027452522

SN - 0014-5793

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EP - 78

JO - FEBS Letters

JF - FEBS Letters

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ER -

Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma

Abstract

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Keywords

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