Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

J. Michael Conlon; Ambikaipakan Balasubramaniam; Stacia A. Sower

doi:10.1016/0167-0115(94)90032-9

Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

J. Michael Conlon, Ambikaipakan Balasubramaniam, Stacia A. Sower

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

Original language	English
Pages (from-to)	167-175
Number of pages	9
Journal	Regulatory Peptides
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0167-0115(94)90032-9
Publication status	Published (in print/issue) - 24 Feb 1994

Keywords

Agnathan
Estradiol
Peptide methionine-tyrosine (PMY)

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10.1016/0167-0115(94)90032-9

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title = "Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis",

abstract = "A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.",

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T1 - Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

AU - Michael Conlon, J.

AU - Balasubramaniam, Ambikaipakan

AU - Sower, Stacia A.

PY - 1994/2/24

Y1 - 1994/2/24

N2 - A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

AB - A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

KW - Agnathan

KW - Estradiol

KW - Peptide methionine-tyrosine (PMY)

UR - https://www.scopus.com/pages/publications/0028267129

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DO - 10.1016/0167-0115(94)90032-9

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AN - SCOPUS:0028267129

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VL - 50

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JO - Regulatory Peptides

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IS - 2

ER -

Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

Abstract

Keywords

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