Abstract
A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.
| Original language | English |
|---|---|
| Pages (from-to) | 167-175 |
| Number of pages | 9 |
| Journal | Regulatory Peptides |
| Volume | 50 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published (in print/issue) - 24 Feb 1994 |
Funding
The authors would like to thank Dr. M.M.T O'Hare, Queen's University of Belfast, N. Ireland for a gift of antiserum and Ms. Kelly Deragon for help with lamprey maintenance. The work was supported by NSF Grants (DCB-9004332, 8904919 and 9117387), NIH Grant GM47122 and the Great Lakes Fishery Commission.
| Funders | Funder number |
|---|---|
| National Science Foundation | 8904919, DCB-9004332, 9117387 |
| National Institutes of Health | |
| R01GM047122 | |
Keywords
- Agnathan
- Estradiol
- Peptide methionine-tyrosine (PMY)
Fingerprint
Dive into the research topics of 'Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver