Purification, Conformational Analysis, and Properties of a Family ofTigerinin Peptides from Skin Secretions of the Crowned BullfrogHoplobatrachus occipitalis

CM McLaughlin, S Lampis, M Mechkarska, L Coquet, T Jouenne, JD King, ML Mangoni, ML Lukic, MA Scorciapino, J. Michael Conlon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Four host-defense peptides belonging to the tigerinin family (tigerinin-1O: RICTPIPFPMCY; tigerinin-2O: RTCIPIPLVMC; tigerinin-3O: RICTAIPLPMCL; and tigerinin- 4O: RTCIPIPPVCF) were isolated from skin secretions of the African crowned bullfrog Hoplobatrachus occipitalis. Inaqueous solution at pH 4.8, the cyclic domain of tigerinin-2O adopts a rigid amphipathic conformation that incorporates a flexible N-terminal tail. The tigerinins lacked antimicrobial (MIC > 100 μM) and hemolytic (LC50 > 500 μM) activities but, at a concentration of 20 μg/mL, significantly (P <0.05) inhibited production of interferon-γ (IFN-γ) by peritoneal cells from C57BL/6 mice without affecting production of IL-10 and IL-17. Tigerinin-2O and -4O inhibited IFN-γ production at concentrations as low as 1 μg/mL. The tigerinins significantly (P ≤ 0.05) stimulated the rate of insulin release from BRIN-BD11 clonal β-cells without compromising the integrity of the plasma membrane. Tigerinin-1O was the most potent (threshold concentration 1 nM) and the most effective (395% increase over basal rate at a concentration of 1 μM). Tigerinin-4O was themost potent and effective peptide in stimulating the rate of glucagon-like peptide-1 release from GLUTag enteroendocrine cells (threshold concentration 10 nM; 289% increase over basal rate at 1 μM). Tigerinin peptides have potential for development into agents for the treatment of patients with type 2 diabetes.
LanguageEnglish
Pages1-7
JournalJournal of Natural Products
VolumeN/A
Early online date25 Aug 2016
DOIs
Publication statusE-pub ahead of print - 25 Aug 2016

Fingerprint

Skin
Peptides
Interferon-gamma
Enteroendocrine Cells
Rana catesbeiana
Glucagon-Like Peptide 1
Interleukin-17
Proxy
Inbred C57BL Mouse
Interleukin-10
Type 2 Diabetes Mellitus
Tail
Cell Membrane
Insulin
Therapeutics

Keywords

  • Tigerinin Peptides
  • natural products
  • Crowned Bullfrog

Cite this

McLaughlin, CM ; Lampis, S ; Mechkarska, M ; Coquet, L ; Jouenne, T ; King, JD ; Mangoni, ML ; Lukic, ML ; Scorciapino, MA ; Conlon, J. Michael. / Purification, Conformational Analysis, and Properties of a Family ofTigerinin Peptides from Skin Secretions of the Crowned BullfrogHoplobatrachus occipitalis. In: Journal of Natural Products. 2016 ; Vol. N/A. pp. 1-7.
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abstract = "Four host-defense peptides belonging to the tigerinin family (tigerinin-1O: RICTPIPFPMCY; tigerinin-2O: RTCIPIPLVMC; tigerinin-3O: RICTAIPLPMCL; and tigerinin- 4O: RTCIPIPPVCF) were isolated from skin secretions of the African crowned bullfrog Hoplobatrachus occipitalis. Inaqueous solution at pH 4.8, the cyclic domain of tigerinin-2O adopts a rigid amphipathic conformation that incorporates a flexible N-terminal tail. The tigerinins lacked antimicrobial (MIC > 100 μM) and hemolytic (LC50 > 500 μM) activities but, at a concentration of 20 μg/mL, significantly (P <0.05) inhibited production of interferon-γ (IFN-γ) by peritoneal cells from C57BL/6 mice without affecting production of IL-10 and IL-17. Tigerinin-2O and -4O inhibited IFN-γ production at concentrations as low as 1 μg/mL. The tigerinins significantly (P ≤ 0.05) stimulated the rate of insulin release from BRIN-BD11 clonal β-cells without compromising the integrity of the plasma membrane. Tigerinin-1O was the most potent (threshold concentration 1 nM) and the most effective (395{\%} increase over basal rate at a concentration of 1 μM). Tigerinin-4O was themost potent and effective peptide in stimulating the rate of glucagon-like peptide-1 release from GLUTag enteroendocrine cells (threshold concentration 10 nM; 289{\%} increase over basal rate at 1 μM). Tigerinin peptides have potential for development into agents for the treatment of patients with type 2 diabetes.",
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Purification, Conformational Analysis, and Properties of a Family ofTigerinin Peptides from Skin Secretions of the Crowned BullfrogHoplobatrachus occipitalis. / McLaughlin, CM; Lampis, S; Mechkarska, M; Coquet, L; Jouenne, T; King, JD; Mangoni, ML; Lukic, ML; Scorciapino, MA; Conlon, J. Michael.

In: Journal of Natural Products, Vol. N/A, 25.08.2016, p. 1-7.

Research output: Contribution to journalArticle

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T1 - Purification, Conformational Analysis, and Properties of a Family ofTigerinin Peptides from Skin Secretions of the Crowned BullfrogHoplobatrachus occipitalis

AU - McLaughlin, CM

AU - Lampis, S

AU - Mechkarska, M

AU - Coquet, L

AU - Jouenne, T

AU - King, JD

AU - Mangoni, ML

AU - Lukic, ML

AU - Scorciapino, MA

AU - Conlon, J. Michael

PY - 2016/8/25

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AB - Four host-defense peptides belonging to the tigerinin family (tigerinin-1O: RICTPIPFPMCY; tigerinin-2O: RTCIPIPLVMC; tigerinin-3O: RICTAIPLPMCL; and tigerinin- 4O: RTCIPIPPVCF) were isolated from skin secretions of the African crowned bullfrog Hoplobatrachus occipitalis. Inaqueous solution at pH 4.8, the cyclic domain of tigerinin-2O adopts a rigid amphipathic conformation that incorporates a flexible N-terminal tail. The tigerinins lacked antimicrobial (MIC > 100 μM) and hemolytic (LC50 > 500 μM) activities but, at a concentration of 20 μg/mL, significantly (P <0.05) inhibited production of interferon-γ (IFN-γ) by peritoneal cells from C57BL/6 mice without affecting production of IL-10 and IL-17. Tigerinin-2O and -4O inhibited IFN-γ production at concentrations as low as 1 μg/mL. The tigerinins significantly (P ≤ 0.05) stimulated the rate of insulin release from BRIN-BD11 clonal β-cells without compromising the integrity of the plasma membrane. Tigerinin-1O was the most potent (threshold concentration 1 nM) and the most effective (395% increase over basal rate at a concentration of 1 μM). Tigerinin-4O was themost potent and effective peptide in stimulating the rate of glucagon-like peptide-1 release from GLUTag enteroendocrine cells (threshold concentration 10 nM; 289% increase over basal rate at 1 μM). Tigerinin peptides have potential for development into agents for the treatment of patients with type 2 diabetes.

KW - Tigerinin Peptides

KW - natural products

KW - Crowned Bullfrog

U2 - 10.1021/acs.jnatprod.6b00494

DO - 10.1021/acs.jnatprod.6b00494

M3 - Article

VL - N/A

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JO - Journal of Natural Products

T2 - Journal of Natural Products

JF - Journal of Natural Products

SN - 0163-3864

ER -