Purification, Conformational Analysis, and Properties of a Family ofTigerinin Peptides from Skin Secretions of the Crowned BullfrogHoplobatrachus occipitalis

CM McLaughlin, S Lampis, M Mechkarska, L Coquet, T Jouenne, JD King, ML Mangoni, ML Lukic, MA Scorciapino, J. Michael Conlon

Research output: Contribution to journalArticle

7 Citations (Scopus)
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Abstract

Four host-defense peptides belonging to the tigerinin family (tigerinin-1O: RICTPIPFPMCY; tigerinin-2O: RTCIPIPLVMC; tigerinin-3O: RICTAIPLPMCL; and tigerinin- 4O: RTCIPIPPVCF) were isolated from skin secretions of the African crowned bullfrog Hoplobatrachus occipitalis. Inaqueous solution at pH 4.8, the cyclic domain of tigerinin-2O adopts a rigid amphipathic conformation that incorporates a flexible N-terminal tail. The tigerinins lacked antimicrobial (MIC > 100 μM) and hemolytic (LC50 > 500 μM) activities but, at a concentration of 20 μg/mL, significantly (P <0.05) inhibited production of interferon-γ (IFN-γ) by peritoneal cells from C57BL/6 mice without affecting production of IL-10 and IL-17. Tigerinin-2O and -4O inhibited IFN-γ production at concentrations as low as 1 μg/mL. The tigerinins significantly (P ≤ 0.05) stimulated the rate of insulin release from BRIN-BD11 clonal β-cells without compromising the integrity of the plasma membrane. Tigerinin-1O was the most potent (threshold concentration 1 nM) and the most effective (395% increase over basal rate at a concentration of 1 μM). Tigerinin-4O was themost potent and effective peptide in stimulating the rate of glucagon-like peptide-1 release from GLUTag enteroendocrine cells (threshold concentration 10 nM; 289% increase over basal rate at 1 μM). Tigerinin peptides have potential for development into agents for the treatment of patients with type 2 diabetes.
Original languageEnglish
Pages (from-to)1-7
JournalJournal of Natural Products
VolumeN/A
Early online date25 Aug 2016
DOIs
Publication statusE-pub ahead of print - 25 Aug 2016

Keywords

  • Tigerinin Peptides
  • natural products
  • Crowned Bullfrog

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