TY - JOUR
T1 - Purification and characterization of galanin and scyliorhinin I from the hybrid sturgeon, Scaphirhynchus platorynchus x Scaphirhynchus albus (Acipenseriformes)
AU - Wang, Yuqi
AU - Barton, Bruce A.
AU - Thim, Lars
AU - Nielsen, Per F.
AU - Conlon, J. Michael
PY - 1999/1
Y1 - 1999/1
N2 - The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu10- Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg20-Ser-Leu-Ser-Asp-Lys-His-Gly-Leu- Pro-NH2) contains only two amino acid substitutions (Ser23→Asn and Pro29→Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe- Tyr-Gly-Leu-Met-NH2) contains only one amino acid substitution (Tyr3→Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
AB - The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu10- Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg20-Ser-Leu-Ser-Asp-Lys-His-Gly-Leu- Pro-NH2) contains only two amino acid substitutions (Ser23→Asn and Pro29→Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe- Tyr-Gly-Leu-Met-NH2) contains only one amino acid substitution (Tyr3→Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
UR - http://www.scopus.com/inward/record.url?scp=0032948144&partnerID=8YFLogxK
U2 - 10.1006/gcen.1998.7174
DO - 10.1006/gcen.1998.7174
M3 - Article
C2 - 9882542
AN - SCOPUS:0032948144
SN - 0016-6480
VL - 113
SP - 38
EP - 45
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -