PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE

HJ Curragh, H Mooibroek, JGH Wessels, R Marchant, E Mullan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.
LanguageEnglish
Pages313-317
JournalMycological Research
Volume97
Issue numberPart 3
Publication statusPublished - Mar 1993

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hygromycin B
Fusarium culmorum
protoplasts
Aspergillus nidulans
DNA
sorbitol
plasmids
phosphotransferases (kinases)
sucrose
Escherichia coli
genes

Cite this

Curragh, HJ., Mooibroek, H., Wessels, JGH., Marchant, R., & Mullan, E. (1993). PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE. Mycological Research, 97(Part 3), 313-317.
Curragh, HJ ; Mooibroek, H ; Wessels, JGH ; Marchant, R ; Mullan, E. / PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE. In: Mycological Research. 1993 ; Vol. 97, No. Part 3. pp. 313-317.
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Curragh, HJ, Mooibroek, H, Wessels, JGH, Marchant, R & Mullan, E 1993, 'PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE', Mycological Research, vol. 97, no. Part 3, pp. 313-317.

PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE. / Curragh, HJ; Mooibroek, H; Wessels, JGH; Marchant, R; Mullan, E.

In: Mycological Research, Vol. 97, No. Part 3, 03.1993, p. 313-317.

Research output: Contribution to journalArticle

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AU - Curragh, HJ

AU - Mooibroek, H

AU - Wessels, JGH

AU - Marchant, R

AU - Mullan, E

PY - 1993/3

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AB - This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.

M3 - Article

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SP - 313

EP - 317

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T2 - Mycological Research

JF - Mycological Research

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