PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE

HJ Curragh; H Mooibroek; JGH Wessels; R Marchant; E Mullan

PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE

HJ Curragh, H Mooibroek, JGH Wessels, R Marchant, E Mullan

Faculty Of Life & Health Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.

Original language	English
Pages (from-to)	313-317
Journal	Mycological Research
Volume	97
Issue number	Part 3
Publication status	Published (in print/issue) - Mar 1993

Cite this

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title = "PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE",

abstract = "This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.",

author = "HJ Curragh and H Mooibroek and JGH Wessels and R Marchant and E Mullan",

year = "1993",

month = mar,

language = "English",

volume = "97",

pages = "313--317",

journal = "Mycological Research",

issn = "1469-8102",

publisher = "Elsevier",

number = "Part 3",

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T1 - PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE

AU - Curragh, HJ

AU - Mooibroek, H

AU - Wessels, JGH

AU - Marchant, R

AU - Mullan, E

PY - 1993/3

Y1 - 1993/3

N2 - This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.

AB - This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.

M3 - Article

SN - 1469-8102

VL - 97

SP - 313

EP - 317

JO - Mycological Research

JF - Mycological Research

IS - Part 3

ER -

PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE

Abstract

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