Proteomic profile of cystic fibrosis sputum cells in adults chronically infected with Pseudomonas aeruginosa

SH Pattison, DS Gibson, E Johnston, S Peacock, K Rivera, MM Tunney, DJ Pappin, JS Elborn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lung disease is the main cause of morbidity and mortality in cystic fibrosis (CF) andinvolves chronic infection and perturbed immune responses. Tissue damage ismediated mostly by extracellular proteases, but other cellular proteins may alsocontribute to damage through their effect on cell activities and/or release into sputumfluid by means of active secretion or cell death.We employed Multidimensional Protein Identification Technology to identify sputumcellular proteins with consistently altered abundance in adults with CF, chronicallyinfected with Pseudomonas aeruginosa, compared to healthy controls. IngenuityPathway Analysis, Gene Ontology, protein abundance and correlation with lungfunction were used to infer their potential clinical significance.Differentially abundant proteins relate to Rho family small GTPase activity, immunecell movement/activation, generation of reactive oxygen species and dysregulation ofcell death and proliferation. Compositional breakdown identified high abundance ofproteins previously associated with neutrophil extracellular traps. Furthermore,negative correlations with lung function were detected for 17 proteins, many of whichhave previously been associated with lung injury.These findings expand current understanding of the mechanisms driving CF lungdisease and identify sputum cellular proteins with potential for use as indicators ofdisease status/prognosis, stratification determinants for treatment prescription or astherapeutic targets.
LanguageEnglish
Pages1-11
JournalEuropean Respiratory Journal
Volume50
Issue number1
Early online date1 Jul 2017
DOIs
Publication statusE-pub ahead of print - 1 Jul 2017

Fingerprint

Sputum
Cystic Fibrosis
Proteomics
Pseudomonas aeruginosa
Proteins
Gene Ontology
Monomeric GTP-Binding Proteins
Lung Injury
Lung Diseases
Prescriptions
Reactive Oxygen Species
Peptide Hydrolases
Cell Death
Technology
Morbidity
Lung
Mortality
Infection

Keywords

  • cystic fibrosis
  • sputum
  • proteomics
  • pseudomonas aeruginosa
  • lung function
  • neutrophil extracellular traps

Cite this

Pattison, SH ; Gibson, DS ; Johnston, E ; Peacock, S ; Rivera, K ; Tunney, MM ; Pappin, DJ ; Elborn, JS. / Proteomic profile of cystic fibrosis sputum cells in adults chronically infected with Pseudomonas aeruginosa. In: European Respiratory Journal. 2017 ; Vol. 50, No. 1. pp. 1-11.
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Proteomic profile of cystic fibrosis sputum cells in adults chronically infected with Pseudomonas aeruginosa. / Pattison, SH; Gibson, DS; Johnston, E; Peacock, S; Rivera, K; Tunney, MM; Pappin, DJ; Elborn, JS.

In: European Respiratory Journal, Vol. 50, No. 1, 01.07.2017, p. 1-11.

Research output: Contribution to journalArticle

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