Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis

David Gibson; Sarah Blelock; Simon Brockbank; Jim Curry; Adrienne Healy; Catherine McAllister; Madeleine Rooney

Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis

David Gibson, Sarah Blelock, Simon Brockbank, Jim Curry, Adrienne Healy, Catherine McAllister, Madeleine Rooney

Faculty Of Life & Health Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Abstract

The synovial proteome was investigated to isolate joint-specific biomarkers that are significantly expressed in Juvenile idiopathic arthritis (JIA) patients displaying recurrent inflammation. These proteins could act as novel therapeutic targets. JIA comprises a poorly understood group of chronic autoimmune diseases with variable clinical presentations and outcomes.Serial synovial fluid and matched plasma samples (n=6 patients) were subjected to protein separation by 2-dimension electrophoreisis (2DE). Focused gels were fixed, stained with colloidal Coomassie, scanned and protein spot intensities quantified by Phoretics software analysis. Pooled subgroup profiles (n=12 patients) were used to highlight joint specific protein spots with expression patterns that correlated with recurrence of joint inflammation. Protein spots were picked, digested by trypsin and extracted. Peptides (n=3) were identified with a mass spectrometer.2DE reveals ~680 spots per gel within the pH 4-7 range for synovial fluid and plasma. On comparison of plasma and synovial ‘master’ gels, a subpopulation of spots uniquely expressed in synovial fluid, were identified. The expression levels of 12 synovial protein spots correlated positively with clinical and laboratory indices of inflammation. Furthermore they were over represented in those patients with recurrent joint effusions (p

Original language	English
Title of host publication	Irish Journal of Medical Science
Publisher	Springer
Pages	1-54
Number of pages	1
Volume	174
Edition	4
Publication status	Published (in print/issue) - 2005
Event	Irish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting - Trinity Centre – St. James’s Hospital Dublin 8 Duration: 1 Jan 2005 → …

Conference

Conference	Irish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting
Period	1/01/05 → …

Keywords

Proteomics
bioinformatics
mass spectrometry
juvenile arthritis

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ISR October 2005 abstractsFinal published version, 27 KB

https://www.ijms.ie/Portals/_IJMS/.../ISR%20October%202005%20abstracts.doc

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Gibson, D., Blelock, S., Brockbank, S., Curry, J., Healy, A., McAllister, C., & Rooney, M. (2005). Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis. In Irish Journal of Medical Science (4 ed., Vol. 174, pp. 1-54). Springer. https://www.ijms.ie/Portals/_IJMS/.../ISR%20October%202005%20abstracts.doc

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title = "Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis",

abstract = "The synovial proteome was investigated to isolate joint-specific biomarkers that are significantly expressed in Juvenile idiopathic arthritis (JIA) patients displaying recurrent inflammation. These proteins could act as novel therapeutic targets. JIA comprises a poorly understood group of chronic autoimmune diseases with variable clinical presentations and outcomes.Serial synovial fluid and matched plasma samples (n=6 patients) were subjected to protein separation by 2-dimension electrophoreisis (2DE). Focused gels were fixed, stained with colloidal Coomassie, scanned and protein spot intensities quantified by Phoretics software analysis. Pooled subgroup profiles (n=12 patients) were used to highlight joint specific protein spots with expression patterns that correlated with recurrence of joint inflammation. Protein spots were picked, digested by trypsin and extracted. Peptides (n=3) were identified with a mass spectrometer.2DE reveals ~680 spots per gel within the pH 4-7 range for synovial fluid and plasma. On comparison of plasma and synovial {\textquoteleft}master{\textquoteright} gels, a subpopulation of spots uniquely expressed in synovial fluid, were identified. The expression levels of 12 synovial protein spots correlated positively with clinical and laboratory indices of inflammation. Furthermore they were over represented in those patients with recurrent joint effusions (p",

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booktitle = "Irish Journal of Medical Science",

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Gibson, D, Blelock, S, Brockbank, S, Curry, J, Healy, A, McAllister, C & Rooney, M 2005, Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis. in Irish Journal of Medical Science. 4 edn, vol. 174, Springer, pp. 1-54, Irish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting, 1/01/05. <https://www.ijms.ie/Portals/_IJMS/.../ISR%20October%202005%20abstracts.doc>

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AU - Gibson, David

AU - Blelock, Sarah

AU - Brockbank, Simon

AU - Curry, Jim

AU - Healy, Adrienne

AU - McAllister, Catherine

AU - Rooney, Madeleine

PY - 2005

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N2 - The synovial proteome was investigated to isolate joint-specific biomarkers that are significantly expressed in Juvenile idiopathic arthritis (JIA) patients displaying recurrent inflammation. These proteins could act as novel therapeutic targets. JIA comprises a poorly understood group of chronic autoimmune diseases with variable clinical presentations and outcomes.Serial synovial fluid and matched plasma samples (n=6 patients) were subjected to protein separation by 2-dimension electrophoreisis (2DE). Focused gels were fixed, stained with colloidal Coomassie, scanned and protein spot intensities quantified by Phoretics software analysis. Pooled subgroup profiles (n=12 patients) were used to highlight joint specific protein spots with expression patterns that correlated with recurrence of joint inflammation. Protein spots were picked, digested by trypsin and extracted. Peptides (n=3) were identified with a mass spectrometer.2DE reveals ~680 spots per gel within the pH 4-7 range for synovial fluid and plasma. On comparison of plasma and synovial ‘master’ gels, a subpopulation of spots uniquely expressed in synovial fluid, were identified. The expression levels of 12 synovial protein spots correlated positively with clinical and laboratory indices of inflammation. Furthermore they were over represented in those patients with recurrent joint effusions (p

AB - The synovial proteome was investigated to isolate joint-specific biomarkers that are significantly expressed in Juvenile idiopathic arthritis (JIA) patients displaying recurrent inflammation. These proteins could act as novel therapeutic targets. JIA comprises a poorly understood group of chronic autoimmune diseases with variable clinical presentations and outcomes.Serial synovial fluid and matched plasma samples (n=6 patients) were subjected to protein separation by 2-dimension electrophoreisis (2DE). Focused gels were fixed, stained with colloidal Coomassie, scanned and protein spot intensities quantified by Phoretics software analysis. Pooled subgroup profiles (n=12 patients) were used to highlight joint specific protein spots with expression patterns that correlated with recurrence of joint inflammation. Protein spots were picked, digested by trypsin and extracted. Peptides (n=3) were identified with a mass spectrometer.2DE reveals ~680 spots per gel within the pH 4-7 range for synovial fluid and plasma. On comparison of plasma and synovial ‘master’ gels, a subpopulation of spots uniquely expressed in synovial fluid, were identified. The expression levels of 12 synovial protein spots correlated positively with clinical and laboratory indices of inflammation. Furthermore they were over represented in those patients with recurrent joint effusions (p

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KW - bioinformatics

KW - mass spectrometry

KW - juvenile arthritis

M3 - Conference contribution

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EP - 54

BT - Irish Journal of Medical Science

PB - Springer

T2 - Irish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting

Y2 - 1 January 2005

ER -

Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis

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