Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis

David Gibson, Sarah Blelock, Simon Brockbank, Jim Curry, Adrienne Healy, Catherine McAllister, Madeleine Rooney

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Abstract

The synovial proteome was investigated to isolate joint-specific biomarkers that are significantly expressed in Juvenile idiopathic arthritis (JIA) patients displaying recurrent inflammation. These proteins could act as novel therapeutic targets. JIA comprises a poorly understood group of chronic autoimmune diseases with variable clinical presentations and outcomes.Serial synovial fluid and matched plasma samples (n=6 patients) were subjected to protein separation by 2-dimension electrophoreisis (2DE). Focused gels were fixed, stained with colloidal Coomassie, scanned and protein spot intensities quantified by Phoretics software analysis. Pooled subgroup profiles (n=12 patients) were used to highlight joint specific protein spots with expression patterns that correlated with recurrence of joint inflammation. Protein spots were picked, digested by trypsin and extracted. Peptides (n=3) were identified with a mass spectrometer.2DE reveals ~680 spots per gel within the pH 4-7 range for synovial fluid and plasma. On comparison of plasma and synovial ‘master’ gels, a subpopulation of spots uniquely expressed in synovial fluid, were identified. The expression levels of 12 synovial protein spots correlated positively with clinical and laboratory indices of inflammation. Furthermore they were over represented in those patients with recurrent joint effusions (p
Original languageEnglish
Title of host publicationIrish Journal of Medical Science
Pages1-54
Number of pages1
Volume174
Edition4
Publication statusPublished - 2005
EventIrish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting - Trinity Centre – St. James’s Hospital Dublin 8
Duration: 1 Jan 2005 → …

Conference

ConferenceIrish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting
Period1/01/05 → …

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Keywords

  • Proteomics
  • bioinformatics
  • mass spectrometry
  • juvenile arthritis

Cite this

Gibson, D., Blelock, S., Brockbank, S., Curry, J., Healy, A., McAllister, C., & Rooney, M. (2005). Proteomic identification of a biomarker cluster associated with recurrent joint inflammation in Juvenile Idiopathic Arthritis. In Irish Journal of Medical Science (4 ed., Vol. 174, pp. 1-54)