Proteomic analysis of the chronically infected CF airways

Sally Pattison; E Johnston; David Gibson; D Pappin; JS Elborn

doi:DOI: 10.1016/S1569-1993(12)60084-8

Proteomic analysis of the chronically infected CF airways

Sally Pattison
, E Johnston
, David Gibson
, D Pappin
, JS Elborn

unknown

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Abstract

Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p

Original language	English
Title of host publication	Journal of Cystic Fibrosis
Publisher	Elsevier
Pages	S26
Number of pages	2
Volume	11
Edition	1
DOIs	https://doi.org/DOI: 10.1016/S1569-1993(12)60084-8
Publication status	Published (in print/issue) - Jun 2012
Event	35th European Cystic Fibrosis Conference - Dublin, Ireland Duration: 1 Jun 2012 → …

Conference

Conference	35th European Cystic Fibrosis Conference
Period	1/06/12 → …

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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DOI: 10.1016/S1569-1993(12)60084-8

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title = "Proteomic analysis of the chronically infected CF airways",

abstract = "Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p",

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TY - GEN

T1 - Proteomic analysis of the chronically infected CF airways

AU - Pattison, Sally

AU - Johnston, E

AU - Gibson, David

AU - Pappin, D

AU - Elborn, JS

PY - 2012/6

Y1 - 2012/6

N2 - Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p

AB - Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p

U2 - DOI: 10.1016/S1569-1993(12)60084-8

DO - DOI: 10.1016/S1569-1993(12)60084-8

M3 - Conference contribution

VL - 11

SP - S26

BT - Journal of Cystic Fibrosis

PB - Elsevier

T2 - 35th European Cystic Fibrosis Conference

Y2 - 1 June 2012

ER -

Proteomic analysis of the chronically infected CF airways

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