Proteomic analysis of the chronically infected CF airways

Sally Pattison, E Johnston, David Gibson, D Pappin, JS Elborn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p
LanguageEnglish
Title of host publicationUnknown Host Publication
PublisherElsevier
Number of pages2
Publication statusPublished - Jun 2012
Event35th European Cystic Fibrosis Conference - Dublin, Ireland
Duration: 1 Jun 2012 → …

Conference

Conference35th European Cystic Fibrosis Conference
Period1/06/12 → …

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Proteomics
Microbiota
Sputum
Pseudomonas aeruginosa
Lung Diseases
Proteins
Biomarkers
Inflammation
Infection

Cite this

Pattison, S., Johnston, E., Gibson, D., Pappin, D., & Elborn, JS. (2012). Proteomic analysis of the chronically infected CF airways. In Unknown Host Publication Elsevier.
Pattison, Sally ; Johnston, E ; Gibson, David ; Pappin, D ; Elborn, JS. / Proteomic analysis of the chronically infected CF airways. Unknown Host Publication. Elsevier, 2012.
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Pattison, S, Johnston, E, Gibson, D, Pappin, D & Elborn, JS 2012, Proteomic analysis of the chronically infected CF airways. in Unknown Host Publication. Elsevier, 35th European Cystic Fibrosis Conference, 1/06/12.

Proteomic analysis of the chronically infected CF airways. / Pattison, Sally; Johnston, E; Gibson, David; Pappin, D; Elborn, JS.

Unknown Host Publication. Elsevier, 2012.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Proteomic analysis of the chronically infected CF airways

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AU - Johnston, E

AU - Gibson, David

AU - Pappin, D

AU - Elborn, JS

PY - 2012/6

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N2 - Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p

AB - Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p

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BT - Unknown Host Publication

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Pattison S, Johnston E, Gibson D, Pappin D, Elborn JS. Proteomic analysis of the chronically infected CF airways. In Unknown Host Publication. Elsevier. 2012