Proteomic analysis of the chronically infected CF airways

Sally Pattison, E Johnston, David Gibson, D Pappin, JS Elborn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Objectives: Progressive lung disease, driven by inflammation secondary to chronicbacterial infection, constitutes the chief burden of CF.We describe a systems biologyinvestigation of the pathogenesis of Pseudomonas aeruginosa infected CF airwaysaiming to identify biomarkers/pathways as potential targets for improved therapyor for use as prognostic indicators.Methods: We employed multi-dimensional LC-MS/MS to semi-quantitatively contrastthe cellular protein profiles of sputum from CF and control cohorts. Importantly,our approach effectively assesses the activity of CF cells directly in theirin vivo environment, so taking into account the interactions between host and thehighly complex CF microbiome.Results: 119 of the 2309 human proteins detected were common to all samples(36 CF + 12 control). Of these, 49 were down-regulated and 29 up-regulatedin CF (p
Original languageEnglish
Title of host publicationJournal of Cystic Fibrosis
PublisherElsevier
PagesS26
Number of pages2
Volume11
Edition1
DOIs
Publication statusPublished - Jun 2012
Event35th European Cystic Fibrosis Conference - Dublin, Ireland
Duration: 1 Jun 2012 → …

Conference

Conference35th European Cystic Fibrosis Conference
Period1/06/12 → …

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    Pattison, S., Johnston, E., Gibson, D., Pappin, D., & Elborn, JS. (2012). Proteomic analysis of the chronically infected CF airways. In Journal of Cystic Fibrosis (1 ed., Vol. 11, pp. S26). Elsevier. https://doi.org/DOI: 10.1016/S1569-1993(12)60084-8