Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis.

David S. Gibson, Sarah Blelock, Simon Brockbank, Jim Curry, Adrienne Healy, Catherine McAllister, Madeleine E. Rooney

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The synovial fluid proteome in juvenile idiopathic arthritis was investigated to isolate joint-specific biomarkers that are expressed in patients displaying recurrent joint inflammation. To identify the synovial specific proteome, matched synovial fluid and plasma samples were subjected to protein separation by 2-dimension electrophoresis (2DE). Forty-three protein spots, overexpressed in the joint, were identified. Synovial fluids from children with single-event knee joint inflammation were then compared with a group with recurrent knee disease. Nine synovial specific proteins were significantly differentially expressed in the recurrent group. Proteolytic fragments of collagen X, fibrin beta-chain, and T-cell receptor alpha-region have been identified among this protein cluster. Putative biomarkers, overexpressed in the joint and differentially expressed in children with recurrent joint inflammation, have been identified. These proteins may play a significant role determining the pathological state within the chronically inflamed joint and influence disease progression in JIA. This is the first study of the synovial proteome in children.
LanguageEnglish
Pages1988-1995
JournalJournal of Proteome Research
Volume5
Issue number8
DOIs
Publication statusPublished - 14 Jul 2006

Fingerprint

Juvenile Arthritis
Proteomics
Joints
Inflammation
Synovial Fluid
Proteome
Antigen Receptors, T-Cell, alpha-beta
Biomarkers
Proteins
Fluids
Joint Diseases
Knee Joint
Fibrin
Electrophoresis
Disease Progression
Knee
Collagen
Plasmas

Keywords

  • Proteome
  • juvenile arthritis
  • inflammation
  • bioinformatics

Cite this

Gibson, D. S., Blelock, S., Brockbank, S., Curry, J., Healy, A., McAllister, C., & Rooney, M. E. (2006). Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis. Journal of Proteome Research, 5(8), 1988-1995. https://doi.org/10.1021/pr060129t
Gibson, David S. ; Blelock, Sarah ; Brockbank, Simon ; Curry, Jim ; Healy, Adrienne ; McAllister, Catherine ; Rooney, Madeleine E. / Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 8. pp. 1988-1995.
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Gibson, DS, Blelock, S, Brockbank, S, Curry, J, Healy, A, McAllister, C & Rooney, ME 2006, 'Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis.', Journal of Proteome Research, vol. 5, no. 8, pp. 1988-1995. https://doi.org/10.1021/pr060129t

Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis. / Gibson, David S.; Blelock, Sarah; Brockbank, Simon; Curry, Jim; Healy, Adrienne; McAllister, Catherine; Rooney, Madeleine E.

In: Journal of Proteome Research, Vol. 5, No. 8, 14.07.2006, p. 1988-1995.

Research output: Contribution to journalArticle

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