Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis.

David S. Gibson, Sarah Blelock, Simon Brockbank, Jim Curry, Adrienne Healy, Catherine McAllister, Madeleine E. Rooney

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21 Citations (Scopus)

Abstract

The synovial fluid proteome in juvenile idiopathic arthritis was investigated to isolate joint-specific biomarkers that are expressed in patients displaying recurrent joint inflammation. To identify the synovial specific proteome, matched synovial fluid and plasma samples were subjected to protein separation by 2-dimension electrophoresis (2DE). Forty-three protein spots, overexpressed in the joint, were identified. Synovial fluids from children with single-event knee joint inflammation were then compared with a group with recurrent knee disease. Nine synovial specific proteins were significantly differentially expressed in the recurrent group. Proteolytic fragments of collagen X, fibrin beta-chain, and T-cell receptor alpha-region have been identified among this protein cluster. Putative biomarkers, overexpressed in the joint and differentially expressed in children with recurrent joint inflammation, have been identified. These proteins may play a significant role determining the pathological state within the chronically inflamed joint and influence disease progression in JIA. This is the first study of the synovial proteome in children.
Original languageEnglish
Pages (from-to)1988-1995
JournalJournal of Proteome Research
Volume5
Issue number8
DOIs
Publication statusPublished - 14 Jul 2006

Keywords

  • Proteome
  • juvenile arthritis
  • inflammation
  • bioinformatics

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    Gibson, D. S., Blelock, S., Brockbank, S., Curry, J., Healy, A., McAllister, C., & Rooney, M. E. (2006). Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis. Journal of Proteome Research, 5(8), 1988-1995. https://doi.org/10.1021/pr060129t