Protein phosphorylation detection using dual-mode field-effect devices and nanoplasmonic sensors

Nikhil Bhalla, Mirella Di Lorenzo, Giordano Pula, Pedro Estrela

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Phosphorylation by kinases is an important post-translational modification of proteins. It is a critical control for the regulation of vital cellular activities, and its dysregulation is implicated in several diseases. A common drug discovery approach involves, therefore, time-consuming screenings of large libraries of candidate compounds to identify novel inhibitors of protein kinases. In this work, we propose a novel method that combines localized surface plasmon resonance (LSPR) and electrolyte insulator semiconductor (EIS)-based proton detection for the rapid identification of novel protein kinase inhibitors. In particular, the selective detection of thiophosphorylated proteins by LSPR is achieved by changing their resonance properties via a pre-binding with gold nanoparticles. In parallel, the EIS field-effect structure allows the real-time electrochemical monitoring of the protein phosphorylation by detecting the release of protons associated with the kinases activity. This innovative combination of both field-effect and nanoplasmonic sensing makes the detection of protein phosphorylation more reliable and effective. As a result, the screening of protein kinase inhibitors becomes more rapid, sensitive, robust and cost-effective.

LanguageEnglish
Article number8687
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 3 Mar 2015

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Protein Kinase Inhibitors
Semiconductors
Surface Plasmon Resonance
Phosphorylation
Equipment and Supplies
Electrolytes
Protons
Phosphotransferases
Proteins
Drug Discovery
Post Translational Protein Processing
Gold
Nanoparticles
Libraries
Costs and Cost Analysis

Cite this

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abstract = "Phosphorylation by kinases is an important post-translational modification of proteins. It is a critical control for the regulation of vital cellular activities, and its dysregulation is implicated in several diseases. A common drug discovery approach involves, therefore, time-consuming screenings of large libraries of candidate compounds to identify novel inhibitors of protein kinases. In this work, we propose a novel method that combines localized surface plasmon resonance (LSPR) and electrolyte insulator semiconductor (EIS)-based proton detection for the rapid identification of novel protein kinase inhibitors. In particular, the selective detection of thiophosphorylated proteins by LSPR is achieved by changing their resonance properties via a pre-binding with gold nanoparticles. In parallel, the EIS field-effect structure allows the real-time electrochemical monitoring of the protein phosphorylation by detecting the release of protons associated with the kinases activity. This innovative combination of both field-effect and nanoplasmonic sensing makes the detection of protein phosphorylation more reliable and effective. As a result, the screening of protein kinase inhibitors becomes more rapid, sensitive, robust and cost-effective.",
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Protein phosphorylation detection using dual-mode field-effect devices and nanoplasmonic sensors. / Bhalla, Nikhil; Di Lorenzo, Mirella; Pula, Giordano; Estrela, Pedro.

In: Scientific Reports, Vol. 5, 8687, 03.03.2015.

Research output: Contribution to journalArticle

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