Prolonged L-alanine exposure induces changes in metabolism, Ca2+ handling and desensitization of insulin secretion in clonal pancreatic beta-cells

Neville McClenaghan, Siobhan M. Scullion, Brian Mion, Chandralal Hewage, J. Paul G. Malthouse, Peter Flatt, Philip Newsholme, Lorraine Brennan

Research output: Contribution to journalArticle

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Abstract

Acute insulin-releasing actions of amino acids have been studied in detail, but comparatively little is known about the beta-cell effects of long-term exposure to amino acids. The present study examined the effects of prolonged exposure of beta-cells to the metabolizable amino acid L-alanine. Basal insulin release or cellular insulin content were not significantly altered by alanine culture, but acute alanine-induced insulin secretion was suppressed by 74% (P <0.001). Acute stimulation of insulin secretion with glucose, KCI or KIC (2-oxoisocaproic acid) following alanine culture was not affected. Acute alanine exposure evoked strong cellular depolarization after control culture, whereas AUC (area under the curve) analysis revealed significant (P <0.01) suppression of this action after culture with alanine. Compared with control cells, prior exposure to alanine also markedly decreased (P <0.01) the acute elevation of [Ca2+](i), (intracellular [Ca2+]) induced by acute alanine exposure. These diminished stimulatory responses were partially restored after 18 h of culture in the absence of alanine, indicating reversible amino-acid-induced desensitization. C-13 NMR spectra revealed that alanine culture increased glutamate labelling at position C4 (by 60%; P <0.01), as a result of an increase in the singlet peak, indicating increased flux through pyruvate dehydrogenase. Consistent with this, protein expression of the pyruvate dehydrogenase kinases PDK2 and PDK4 was significantly reduced. This was accompanied by a decrease in cellular ATP (P <0.05), consistent with diminished insulin-releasing actions of this amino acid. Collectively, these results illustrate the phenomenon of beta-cell desensitization by amino acids, indicating that prolonged exposure to alanine can induce reversible alterations to metabolic flux, Ca2+ handling and insulin secretion.
LanguageEnglish
Pages341-351
JournalClinical Science
Volume116
Issue number3-4
DOIs
Publication statusPublished - Feb 2009

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Insulin-Secreting Cells
Alanine
Insulin
Amino Acids
Pyruvic Acid
Area Under Curve
Glutamic Acid
Oxidoreductases
Adenosine Triphosphate
Glucose

Cite this

McClenaghan, Neville ; Scullion, Siobhan M. ; Mion, Brian ; Hewage, Chandralal ; Malthouse, J. Paul G. ; Flatt, Peter ; Newsholme, Philip ; Brennan, Lorraine. / Prolonged L-alanine exposure induces changes in metabolism, Ca2+ handling and desensitization of insulin secretion in clonal pancreatic beta-cells. In: Clinical Science. 2009 ; Vol. 116, No. 3-4. pp. 341-351.
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Prolonged L-alanine exposure induces changes in metabolism, Ca2+ handling and desensitization of insulin secretion in clonal pancreatic beta-cells. / McClenaghan, Neville; Scullion, Siobhan M.; Mion, Brian; Hewage, Chandralal; Malthouse, J. Paul G.; Flatt, Peter; Newsholme, Philip; Brennan, Lorraine.

In: Clinical Science, Vol. 116, No. 3-4, 02.2009, p. 341-351.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prolonged L-alanine exposure induces changes in metabolism, Ca2+ handling and desensitization of insulin secretion in clonal pancreatic beta-cells

AU - McClenaghan, Neville

AU - Scullion, Siobhan M.

AU - Mion, Brian

AU - Hewage, Chandralal

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AU - Flatt, Peter

AU - Newsholme, Philip

AU - Brennan, Lorraine

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N2 - Acute insulin-releasing actions of amino acids have been studied in detail, but comparatively little is known about the beta-cell effects of long-term exposure to amino acids. The present study examined the effects of prolonged exposure of beta-cells to the metabolizable amino acid L-alanine. Basal insulin release or cellular insulin content were not significantly altered by alanine culture, but acute alanine-induced insulin secretion was suppressed by 74% (P <0.001). Acute stimulation of insulin secretion with glucose, KCI or KIC (2-oxoisocaproic acid) following alanine culture was not affected. Acute alanine exposure evoked strong cellular depolarization after control culture, whereas AUC (area under the curve) analysis revealed significant (P <0.01) suppression of this action after culture with alanine. Compared with control cells, prior exposure to alanine also markedly decreased (P <0.01) the acute elevation of [Ca2+](i), (intracellular [Ca2+]) induced by acute alanine exposure. These diminished stimulatory responses were partially restored after 18 h of culture in the absence of alanine, indicating reversible amino-acid-induced desensitization. C-13 NMR spectra revealed that alanine culture increased glutamate labelling at position C4 (by 60%; P <0.01), as a result of an increase in the singlet peak, indicating increased flux through pyruvate dehydrogenase. Consistent with this, protein expression of the pyruvate dehydrogenase kinases PDK2 and PDK4 was significantly reduced. This was accompanied by a decrease in cellular ATP (P <0.05), consistent with diminished insulin-releasing actions of this amino acid. Collectively, these results illustrate the phenomenon of beta-cell desensitization by amino acids, indicating that prolonged exposure to alanine can induce reversible alterations to metabolic flux, Ca2+ handling and insulin secretion.

AB - Acute insulin-releasing actions of amino acids have been studied in detail, but comparatively little is known about the beta-cell effects of long-term exposure to amino acids. The present study examined the effects of prolonged exposure of beta-cells to the metabolizable amino acid L-alanine. Basal insulin release or cellular insulin content were not significantly altered by alanine culture, but acute alanine-induced insulin secretion was suppressed by 74% (P <0.001). Acute stimulation of insulin secretion with glucose, KCI or KIC (2-oxoisocaproic acid) following alanine culture was not affected. Acute alanine exposure evoked strong cellular depolarization after control culture, whereas AUC (area under the curve) analysis revealed significant (P <0.01) suppression of this action after culture with alanine. Compared with control cells, prior exposure to alanine also markedly decreased (P <0.01) the acute elevation of [Ca2+](i), (intracellular [Ca2+]) induced by acute alanine exposure. These diminished stimulatory responses were partially restored after 18 h of culture in the absence of alanine, indicating reversible amino-acid-induced desensitization. C-13 NMR spectra revealed that alanine culture increased glutamate labelling at position C4 (by 60%; P <0.01), as a result of an increase in the singlet peak, indicating increased flux through pyruvate dehydrogenase. Consistent with this, protein expression of the pyruvate dehydrogenase kinases PDK2 and PDK4 was significantly reduced. This was accompanied by a decrease in cellular ATP (P <0.05), consistent with diminished insulin-releasing actions of this amino acid. Collectively, these results illustrate the phenomenon of beta-cell desensitization by amino acids, indicating that prolonged exposure to alanine can induce reversible alterations to metabolic flux, Ca2+ handling and insulin secretion.

U2 - 10.1042/CS20080138

DO - 10.1042/CS20080138

M3 - Article

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SP - 341

EP - 351

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SN - 0143-5221

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