Profiling of phenols in human fecal water after raspberry supplementation.

C.I.R Gill, Gordon J McDougall, Sheila Glidewell, Derek Stewart, Qing Shen, Kieran Tuohy, Aine Dobbin, Adele Boyd, Emma Brown, Sumanto Haldar, Ian R Rowland

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    40 Citations (Scopus)

    Abstract

    The phenolic compositions of fecal water samples from ten free-living human subjects without marked dietary restrictions were monitored before and after intake of raspberry puree (200 g/day, 4 days) using gas chromatography-mass spectrometry. No single phenolic component was increased in all subjects after intake, but a majority of subjects had significant elevations in phenylacetic acid (7/10), 4-hydroxyphenylacetic acid (6/10), 3-hydroxyphenylacetic acid (5/10), 3-phenylpropionic acid and 3-(4-hydroxyphenyl)propionic acid. The levels of 3,4-dihydroxbenzoic acid were elevated in 8/10 subjects, significantly for 6 subjects (p <0.05), and not significantly reduced in the other 2 subjects. In addition, unlike most other fecal metabolites, the increase was always >2-fold. This metabolite may be representative of the increased colonic dose of cyanidin anthocyanins. The colonic microbiota varied greatly between individuals, and supplementation with raspberries did not produce any statistically significant alterations in the profile of colonic bacteria, nor was a common pattern revealed to account for the interindividual variations observed in the fecal water phenolic profiles.
    LanguageEnglish
    Pages10389-95
    JournalJournal of Agricultural and Food Chemistry
    Volume58
    Issue number19
    Publication statusPublished - 2010

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    Phenols
    Anthocyanins
    Water
    Microbiota
    Gas Chromatography-Mass Spectrometry
    Bacteria
    Acids
    Rubus
    3-phenylpropionic acid
    phenylacetic acid
    4-hydroxyphenylacetic acid
    3-hydroxybenzeneacetic acid
    cyanidin
    phloretic acid

    Cite this

    Gill, C. I. R., McDougall, G. J., Glidewell, S., Stewart, D., Shen, Q., Tuohy, K., ... Rowland, I. R. (2010). Profiling of phenols in human fecal water after raspberry supplementation. Journal of Agricultural and Food Chemistry, 58(19), 10389-95.
    Gill, C.I.R ; McDougall, Gordon J ; Glidewell, Sheila ; Stewart, Derek ; Shen, Qing ; Tuohy, Kieran ; Dobbin, Aine ; Boyd, Adele ; Brown, Emma ; Haldar, Sumanto ; Rowland, Ian R. / Profiling of phenols in human fecal water after raspberry supplementation. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 19. pp. 10389-95.
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    abstract = "The phenolic compositions of fecal water samples from ten free-living human subjects without marked dietary restrictions were monitored before and after intake of raspberry puree (200 g/day, 4 days) using gas chromatography-mass spectrometry. No single phenolic component was increased in all subjects after intake, but a majority of subjects had significant elevations in phenylacetic acid (7/10), 4-hydroxyphenylacetic acid (6/10), 3-hydroxyphenylacetic acid (5/10), 3-phenylpropionic acid and 3-(4-hydroxyphenyl)propionic acid. The levels of 3,4-dihydroxbenzoic acid were elevated in 8/10 subjects, significantly for 6 subjects (p <0.05), and not significantly reduced in the other 2 subjects. In addition, unlike most other fecal metabolites, the increase was always >2-fold. This metabolite may be representative of the increased colonic dose of cyanidin anthocyanins. The colonic microbiota varied greatly between individuals, and supplementation with raspberries did not produce any statistically significant alterations in the profile of colonic bacteria, nor was a common pattern revealed to account for the interindividual variations observed in the fecal water phenolic profiles.",
    author = "C.I.R Gill and McDougall, {Gordon J} and Sheila Glidewell and Derek Stewart and Qing Shen and Kieran Tuohy and Aine Dobbin and Adele Boyd and Emma Brown and Sumanto Haldar and Rowland, {Ian R}",
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    Gill, CIR, McDougall, GJ, Glidewell, S, Stewart, D, Shen, Q, Tuohy, K, Dobbin, A, Boyd, A, Brown, E, Haldar, S & Rowland, IR 2010, 'Profiling of phenols in human fecal water after raspberry supplementation.', Journal of Agricultural and Food Chemistry, vol. 58, no. 19, pp. 10389-95.

    Profiling of phenols in human fecal water after raspberry supplementation. / Gill, C.I.R; McDougall, Gordon J; Glidewell, Sheila; Stewart, Derek; Shen, Qing; Tuohy, Kieran; Dobbin, Aine; Boyd, Adele; Brown, Emma; Haldar, Sumanto; Rowland, Ian R.

    In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 19, 2010, p. 10389-95.

    Research output: Contribution to journalArticle

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    AU - Gill, C.I.R

    AU - McDougall, Gordon J

    AU - Glidewell, Sheila

    AU - Stewart, Derek

    AU - Shen, Qing

    AU - Tuohy, Kieran

    AU - Dobbin, Aine

    AU - Boyd, Adele

    AU - Brown, Emma

    AU - Haldar, Sumanto

    AU - Rowland, Ian R

    PY - 2010

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    N2 - The phenolic compositions of fecal water samples from ten free-living human subjects without marked dietary restrictions were monitored before and after intake of raspberry puree (200 g/day, 4 days) using gas chromatography-mass spectrometry. No single phenolic component was increased in all subjects after intake, but a majority of subjects had significant elevations in phenylacetic acid (7/10), 4-hydroxyphenylacetic acid (6/10), 3-hydroxyphenylacetic acid (5/10), 3-phenylpropionic acid and 3-(4-hydroxyphenyl)propionic acid. The levels of 3,4-dihydroxbenzoic acid were elevated in 8/10 subjects, significantly for 6 subjects (p <0.05), and not significantly reduced in the other 2 subjects. In addition, unlike most other fecal metabolites, the increase was always >2-fold. This metabolite may be representative of the increased colonic dose of cyanidin anthocyanins. The colonic microbiota varied greatly between individuals, and supplementation with raspberries did not produce any statistically significant alterations in the profile of colonic bacteria, nor was a common pattern revealed to account for the interindividual variations observed in the fecal water phenolic profiles.

    AB - The phenolic compositions of fecal water samples from ten free-living human subjects without marked dietary restrictions were monitored before and after intake of raspberry puree (200 g/day, 4 days) using gas chromatography-mass spectrometry. No single phenolic component was increased in all subjects after intake, but a majority of subjects had significant elevations in phenylacetic acid (7/10), 4-hydroxyphenylacetic acid (6/10), 3-hydroxyphenylacetic acid (5/10), 3-phenylpropionic acid and 3-(4-hydroxyphenyl)propionic acid. The levels of 3,4-dihydroxbenzoic acid were elevated in 8/10 subjects, significantly for 6 subjects (p <0.05), and not significantly reduced in the other 2 subjects. In addition, unlike most other fecal metabolites, the increase was always >2-fold. This metabolite may be representative of the increased colonic dose of cyanidin anthocyanins. The colonic microbiota varied greatly between individuals, and supplementation with raspberries did not produce any statistically significant alterations in the profile of colonic bacteria, nor was a common pattern revealed to account for the interindividual variations observed in the fecal water phenolic profiles.

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    Gill CIR, McDougall GJ, Glidewell S, Stewart D, Shen Q, Tuohy K et al. Profiling of phenols in human fecal water after raspberry supplementation. Journal of Agricultural and Food Chemistry. 2010;58(19):10389-95.