PRODUCTION OF THE ENZYME DIHYDROFOLATE-REDUCTASE BY METHOTREXATE-RESISTANT BACTERIA ISOLATED FROM SOIL

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Abstract

Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR-1 and 3, developed resistance to 500 mug cm-3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1. 5.1. 3): 2580 and 2702 U dm-3 compared to the sensitive parent strains (28 and 35 U dm-3). Isolate PFR-3 showed maximum enzyme production (4950 U dm-3, specific activity 12.56 U mg-1 in flasks and 5737 U dm-3, specific activity 14.80 U mg-1 in 5-dm3 fermenter) during exponential phase of growth (6 h) at 37-degrees-C and pH 7.0.
LanguageEnglish
Pages35-40
JournalJournal of Chemical Technology and Biotechnology
Volume56
Issue number1
Publication statusPublished - 1993

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dihydrofolate reductase
methotrexate
fermenters
bacteria
oxidoreductases
enzymes
soil
microbial culture

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@article{48919fcce6ca459987d090a98d0b7f62,
title = "PRODUCTION OF THE ENZYME DIHYDROFOLATE-REDUCTASE BY METHOTREXATE-RESISTANT BACTERIA ISOLATED FROM SOIL",
abstract = "Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR-1 and 3, developed resistance to 500 mug cm-3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1. 5.1. 3): 2580 and 2702 U dm-3 compared to the sensitive parent strains (28 and 35 U dm-3). Isolate PFR-3 showed maximum enzyme production (4950 U dm-3, specific activity 12.56 U mg-1 in flasks and 5737 U dm-3, specific activity 14.80 U mg-1 in 5-dm3 fermenter) during exponential phase of growth (6 h) at 37-degrees-C and pH 7.0.",
author = "{Singh - Nee Nigam}, Poonam and R Marchant",
year = "1993",
language = "English",
volume = "56",
pages = "35--40",
journal = "Journal of Chemical Technology and Biotechnology",
issn = "0268-2575",
number = "1",

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TY - JOUR

T1 - PRODUCTION OF THE ENZYME DIHYDROFOLATE-REDUCTASE BY METHOTREXATE-RESISTANT BACTERIA ISOLATED FROM SOIL

AU - Singh - Nee Nigam, Poonam

AU - Marchant, R

PY - 1993

Y1 - 1993

N2 - Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR-1 and 3, developed resistance to 500 mug cm-3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1. 5.1. 3): 2580 and 2702 U dm-3 compared to the sensitive parent strains (28 and 35 U dm-3). Isolate PFR-3 showed maximum enzyme production (4950 U dm-3, specific activity 12.56 U mg-1 in flasks and 5737 U dm-3, specific activity 14.80 U mg-1 in 5-dm3 fermenter) during exponential phase of growth (6 h) at 37-degrees-C and pH 7.0.

AB - Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR-1 and 3, developed resistance to 500 mug cm-3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1. 5.1. 3): 2580 and 2702 U dm-3 compared to the sensitive parent strains (28 and 35 U dm-3). Isolate PFR-3 showed maximum enzyme production (4950 U dm-3, specific activity 12.56 U mg-1 in flasks and 5737 U dm-3, specific activity 14.80 U mg-1 in 5-dm3 fermenter) during exponential phase of growth (6 h) at 37-degrees-C and pH 7.0.

M3 - Article

VL - 56

SP - 35

EP - 40

JO - Journal of Chemical Technology and Biotechnology

T2 - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 1

ER -