PRODUCTION OF SALICYLATE HYDROXYLASE FROM PSEUDOMONAS-PUTIDA UUC-1 AND ITS APPLICATION IN THE CONSTRUCTION OF A BIOSENSOR

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Abstract

The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).
Original languageEnglish
Pages (from-to)331-338
JournalJournal of Chemical Technology and Biotechnology
Volume64
Issue number4
Publication statusPublished - Dec 1995

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salicylate 1-monooxygenase
Biosensors
Carbon
Enzyme electrodes
Salicylates
Electrodes
Enzymes
Ointments
Fermentation
Blood

Cite this

@article{daedb10b530848399aaff6ec00026013,
title = "PRODUCTION OF SALICYLATE HYDROXYLASE FROM PSEUDOMONAS-PUTIDA UUC-1 AND ITS APPLICATION IN THE CONSTRUCTION OF A BIOSENSOR",
abstract = "The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).",
author = "DM Zhou and {Singh - Nee Nigam}, Poonam and J Jones and Roger Marchant",
year = "1995",
month = "12",
language = "English",
volume = "64",
pages = "331--338",
journal = "Journal of Chemical Technology and Biotechnology",
issn = "0268-2575",
number = "4",

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T1 - PRODUCTION OF SALICYLATE HYDROXYLASE FROM PSEUDOMONAS-PUTIDA UUC-1 AND ITS APPLICATION IN THE CONSTRUCTION OF A BIOSENSOR

AU - Zhou, DM

AU - Singh - Nee Nigam, Poonam

AU - Jones, J

AU - Marchant, Roger

PY - 1995/12

Y1 - 1995/12

N2 - The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).

AB - The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).

M3 - Article

VL - 64

SP - 331

EP - 338

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 4

ER -