TY - JOUR
T1 - PRODUCTION OF SALICYLATE HYDROXYLASE FROM PSEUDOMONAS-PUTIDA UUC-1 AND ITS APPLICATION IN THE CONSTRUCTION OF A BIOSENSOR
AU - Zhou, DM
AU - Singh - Nee Nigam, Poonam
AU - Jones, J
AU - Marchant, Roger
PY - 1995/12
Y1 - 1995/12
N2 - The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).
AB - The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).
M3 - Article
SN - 1097-4660
VL - 64
SP - 331
EP - 338
JO - Journal of Chemical Technology and Biotechnology
JF - Journal of Chemical Technology and Biotechnology
IS - 4
ER -