PRODUCTION OF INTERLEUKIN-1 BY HIGHLY PURIFIED MONOCYTES IN INFLAMMATORY BOWEL-DISEASE

Y SUZUKI, Daniel Quinn, A TOBIN, CA WHELAN, C OMORAIN

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    Abstract

    In vitro interleukin 1 (IL-1) production by stimulated peripheral blood monocytes, from patients with active Crohn's disease and ulcerative colitis, has been investigated using an ELISA specific for IL-1-beta and a thymocyte proliferation bioassay. Twenty-one patients with active untreated Crohn's disease, 20 with active untreated ulcerative colitis and 10 healthy controls were studied. IL-1 production was significantly increased in Crohn's disease compared with controls by ELISA (11030 +/- 5027 pg/10(6) cells versus 5727 +/- 3106 pg/10(6) cells; P < 0.005) and bioassay [4018 +/- 1770 counts per min (cpm) versus 2250 +/- 700 cpm; P < 0.001]. In contrast, levels of IL-1 production by monocytes from ulcerative colitis were not significantly higher than controls in either the ELISA, or the bioassay [9192 +/- 5958 pg/10(6) cells and 2777 +/- 970 cpm, respectively. Increased production of IL-1 in Crohn's disease may reflect an intrinsic abnormality of monocyte function; alternatively monocyte activation may occur as a consequence of an immune response to an ingested antigen. Increased IL-1 secretion by monocytes may have an important pathogenic role in Crohn's disease.
    LanguageEnglish
    Pages45-49
    JournalEuropean Journal of Gastroenterology and Hepatology
    Volume3
    Issue number1
    Publication statusPublished - Jan 1991

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    Interleukin-1
    Inflammatory Bowel Diseases
    Crohn Disease
    Monocytes
    Ulcerative Colitis
    Biological Assay
    Enzyme-Linked Immunosorbent Assay
    Thymocytes
    Interleukin-1beta
    Antigens

    Cite this

    SUZUKI, Y ; Quinn, Daniel ; TOBIN, A ; WHELAN, CA ; OMORAIN, C. / PRODUCTION OF INTERLEUKIN-1 BY HIGHLY PURIFIED MONOCYTES IN INFLAMMATORY BOWEL-DISEASE. In: European Journal of Gastroenterology and Hepatology. 1991 ; Vol. 3, No. 1. pp. 45-49.
    @article{50ae8ea2e5804de187788c7b6ff20f35,
    title = "PRODUCTION OF INTERLEUKIN-1 BY HIGHLY PURIFIED MONOCYTES IN INFLAMMATORY BOWEL-DISEASE",
    abstract = "In vitro interleukin 1 (IL-1) production by stimulated peripheral blood monocytes, from patients with active Crohn's disease and ulcerative colitis, has been investigated using an ELISA specific for IL-1-beta and a thymocyte proliferation bioassay. Twenty-one patients with active untreated Crohn's disease, 20 with active untreated ulcerative colitis and 10 healthy controls were studied. IL-1 production was significantly increased in Crohn's disease compared with controls by ELISA (11030 +/- 5027 pg/10(6) cells versus 5727 +/- 3106 pg/10(6) cells; P < 0.005) and bioassay [4018 +/- 1770 counts per min (cpm) versus 2250 +/- 700 cpm; P < 0.001]. In contrast, levels of IL-1 production by monocytes from ulcerative colitis were not significantly higher than controls in either the ELISA, or the bioassay [9192 +/- 5958 pg/10(6) cells and 2777 +/- 970 cpm, respectively. Increased production of IL-1 in Crohn's disease may reflect an intrinsic abnormality of monocyte function; alternatively monocyte activation may occur as a consequence of an immune response to an ingested antigen. Increased IL-1 secretion by monocytes may have an important pathogenic role in Crohn's disease.",
    author = "Y SUZUKI and Daniel Quinn and A TOBIN and CA WHELAN and C OMORAIN",
    year = "1991",
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    SUZUKI, Y, Quinn, D, TOBIN, A, WHELAN, CA & OMORAIN, C 1991, 'PRODUCTION OF INTERLEUKIN-1 BY HIGHLY PURIFIED MONOCYTES IN INFLAMMATORY BOWEL-DISEASE', European Journal of Gastroenterology and Hepatology, vol. 3, no. 1, pp. 45-49.

    PRODUCTION OF INTERLEUKIN-1 BY HIGHLY PURIFIED MONOCYTES IN INFLAMMATORY BOWEL-DISEASE. / SUZUKI, Y; Quinn, Daniel; TOBIN, A; WHELAN, CA; OMORAIN, C.

    In: European Journal of Gastroenterology and Hepatology, Vol. 3, No. 1, 01.1991, p. 45-49.

    Research output: Contribution to journalArticle

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    AU - SUZUKI, Y

    AU - Quinn, Daniel

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    AU - WHELAN, CA

    AU - OMORAIN, C

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    Y1 - 1991/1

    N2 - In vitro interleukin 1 (IL-1) production by stimulated peripheral blood monocytes, from patients with active Crohn's disease and ulcerative colitis, has been investigated using an ELISA specific for IL-1-beta and a thymocyte proliferation bioassay. Twenty-one patients with active untreated Crohn's disease, 20 with active untreated ulcerative colitis and 10 healthy controls were studied. IL-1 production was significantly increased in Crohn's disease compared with controls by ELISA (11030 +/- 5027 pg/10(6) cells versus 5727 +/- 3106 pg/10(6) cells; P < 0.005) and bioassay [4018 +/- 1770 counts per min (cpm) versus 2250 +/- 700 cpm; P < 0.001]. In contrast, levels of IL-1 production by monocytes from ulcerative colitis were not significantly higher than controls in either the ELISA, or the bioassay [9192 +/- 5958 pg/10(6) cells and 2777 +/- 970 cpm, respectively. Increased production of IL-1 in Crohn's disease may reflect an intrinsic abnormality of monocyte function; alternatively monocyte activation may occur as a consequence of an immune response to an ingested antigen. Increased IL-1 secretion by monocytes may have an important pathogenic role in Crohn's disease.

    AB - In vitro interleukin 1 (IL-1) production by stimulated peripheral blood monocytes, from patients with active Crohn's disease and ulcerative colitis, has been investigated using an ELISA specific for IL-1-beta and a thymocyte proliferation bioassay. Twenty-one patients with active untreated Crohn's disease, 20 with active untreated ulcerative colitis and 10 healthy controls were studied. IL-1 production was significantly increased in Crohn's disease compared with controls by ELISA (11030 +/- 5027 pg/10(6) cells versus 5727 +/- 3106 pg/10(6) cells; P < 0.005) and bioassay [4018 +/- 1770 counts per min (cpm) versus 2250 +/- 700 cpm; P < 0.001]. In contrast, levels of IL-1 production by monocytes from ulcerative colitis were not significantly higher than controls in either the ELISA, or the bioassay [9192 +/- 5958 pg/10(6) cells and 2777 +/- 970 cpm, respectively. Increased production of IL-1 in Crohn's disease may reflect an intrinsic abnormality of monocyte function; alternatively monocyte activation may occur as a consequence of an immune response to an ingested antigen. Increased IL-1 secretion by monocytes may have an important pathogenic role in Crohn's disease.

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