PRODUCTION OF ETHANOL BY THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA

D BRADY, R Marchant, L MCHALE, Anthony McHale

Research output: Contribution to journalArticle

Abstract

The thermotolerant yeast strain, Kluyveromyces marxianus IMB3 was shown to be capable of growth and ethanol production on lactose containing media at 45 degrees C. On media containing 4% (w/v) lactose, ethanol production increased to 6.0g/l within 50h and this represented 29% of theoretical yield. During growth on lactose containing media the organism was shown to produce a cell-associated beta-galactosidase and no significant enzyme could be detected in the extracellular culture filtrate. Addition of beta-galactosidase, released from Kluyveromyces marxianus IMB3 cells, to active fermentations, resulted in increasing ethanol production to 53% of theoretical yield at 45 degrees C.
LanguageEnglish
Pages737-740
JournalBiotechnology Letters
Volume16
Issue number7
Publication statusPublished - Jul 1994

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Kluyveromyces marxianus
ethanol production
lactose
beta-galactosidase
yeasts
culture filtrates
fermentation
cells
organisms
enzymes

Cite this

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title = "PRODUCTION OF ETHANOL BY THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA",
abstract = "The thermotolerant yeast strain, Kluyveromyces marxianus IMB3 was shown to be capable of growth and ethanol production on lactose containing media at 45 degrees C. On media containing 4{\%} (w/v) lactose, ethanol production increased to 6.0g/l within 50h and this represented 29{\%} of theoretical yield. During growth on lactose containing media the organism was shown to produce a cell-associated beta-galactosidase and no significant enzyme could be detected in the extracellular culture filtrate. Addition of beta-galactosidase, released from Kluyveromyces marxianus IMB3 cells, to active fermentations, resulted in increasing ethanol production to 53{\%} of theoretical yield at 45 degrees C.",
author = "D BRADY and R Marchant and L MCHALE and Anthony McHale",
year = "1994",
month = "7",
language = "English",
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PRODUCTION OF ETHANOL BY THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA. / BRADY, D; Marchant, R; MCHALE, L; McHale, Anthony.

In: Biotechnology Letters, Vol. 16, No. 7, 07.1994, p. 737-740.

Research output: Contribution to journalArticle

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AU - MCHALE, L

AU - McHale, Anthony

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