PRODUCTION OF ETHANOL BY THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA

D BRADY, R MARCHANT, L MCHALE, AP MCHALE

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35 Citations (Scopus)

Abstract

The thermotolerant yeast strain, Kluyveromyces marxianus IMB3 was shown to be capable of growth and ethanol production on lactose containing media at 45 degrees C. On media containing 4% (w/v) lactose, ethanol production increased to 6.0g/l within 50h and this represented 29% of theoretical yield. During growth on lactose containing media the organism was shown to produce a cell-associated beta-galactosidase and no significant enzyme could be detected in the extracellular culture filtrate. Addition of beta-galactosidase, released from Kluyveromyces marxianus IMB3 cells, to active fermentations, resulted in increasing ethanol production to 53% of theoretical yield at 45 degrees C.
Original languageEnglish
Pages (from-to)737-740
JournalBiotechnology Letters
Volume16
Issue number7
DOIs
Publication statusPublished (in print/issue) - Jul 1994

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