Production of cellulase and beta-glucosidase activity during growth of the actinomycete Micromonospora chalcae on cellulose-containing media.

J Gallagher, A Winters, N Barron, L McHale, Anthony McHale

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11 Citations (Scopus)

Abstract

The actinomycete, Micromonospora chalcae produced an extracellular cellulase system when grown on cellulose-containing media. Cellulase activity reached a maximum after 8 days and the beta-glucosidase activity was maximal within 16 to 18 days. beta-Glucosidase activity was isolated and had a Km of 0.25mM for the substrate p-nitrophenyl-beta-D-glucoside. The activity was optimal at pH 7.25 and 52 degrees C.
Original languageEnglish
Pages (from-to)537-540
JournalBiotechnology Letters
Volume18
Issue number5
Publication statusPublished - May 1996

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