The actinomycete, Micromonospora chalcae produced an extracellular cellulase system when grown on cellulose-containing media. Cellulase activity reached a maximum after 8 days and the beta-glucosidase activity was maximal within 16 to 18 days. beta-Glucosidase activity was isolated and had a Km of 0.25mM for the substrate p-nitrophenyl-beta-D-glucoside. The activity was optimal at pH 7.25 and 52 degrees C.
|Publication status||Published - May 1996|
Gallagher, J., Winters, A., Barron, N., McHale, L., & McHale, A. (1996). Production of cellulase and beta-glucosidase activity during growth of the actinomycete Micromonospora chalcae on cellulose-containing media. Biotechnology Letters, 18(5), 537-540.