Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources

R. Thavasi, S. Jayalakshmi, T. Balasubramanian, Ibrahim Banat

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Criteria selected for screening of biosurfactant production by Bacillus megaterium were hemolytic assay, bacterial cell hydrophobicity and the drop-collapse test. The data on hemolytic activity, bacterial cell adherence with crude oil and the drop-collapse test confirmed the biosurfactant-producing ability of the strain. Accordingly, the strain was cultured at different temperatures, pH values, salinity and substrate (crude oil) concentration in mineral salt medium to establish the optimum culture conditions, and it was shown that 38C, 2.0% of substrate concentration, pH 8.0 and 30 parts per thousand of salt concentration were optimal for maximum growth and biosurfactant production. Laboratory scale biosurfactant production in a fermentor was done with crude oil and cheaper carbon sources like waste motor lubricant oil and peanut oil cake, and the highest biosurfactant production was found with peanut oil cake. Characterization of partially purified biosurfactant inferred that it was a glycolipid with emulsification potential of waste motor lubricant oil, crude oil, peanut oil, diesel, kerosene, naphthalene, anthracene and xylene.
LanguageEnglish
Pages917-925
JournalWorld Journal of Microbiology and Biotechnology
Volume24
Issue number7
DOIs
Publication statusPublished - Jul 2008

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biosurfactants
Bacillus megaterium
glycolipids
oils
peanut cake
lubricants
kerosene
xylene
peanut oil
naphthalene
fermenters
emulsifying
hydrophobicity
salt concentration
microbial activity
mineral content
testing
cells
salinity
screening

Cite this

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title = "Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources",
abstract = "Criteria selected for screening of biosurfactant production by Bacillus megaterium were hemolytic assay, bacterial cell hydrophobicity and the drop-collapse test. The data on hemolytic activity, bacterial cell adherence with crude oil and the drop-collapse test confirmed the biosurfactant-producing ability of the strain. Accordingly, the strain was cultured at different temperatures, pH values, salinity and substrate (crude oil) concentration in mineral salt medium to establish the optimum culture conditions, and it was shown that 38C, 2.0{\%} of substrate concentration, pH 8.0 and 30 parts per thousand of salt concentration were optimal for maximum growth and biosurfactant production. Laboratory scale biosurfactant production in a fermentor was done with crude oil and cheaper carbon sources like waste motor lubricant oil and peanut oil cake, and the highest biosurfactant production was found with peanut oil cake. Characterization of partially purified biosurfactant inferred that it was a glycolipid with emulsification potential of waste motor lubricant oil, crude oil, peanut oil, diesel, kerosene, naphthalene, anthracene and xylene.",
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Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources. / Thavasi, R.; Jayalakshmi, S.; Balasubramanian, T.; Banat, Ibrahim.

In: World Journal of Microbiology and Biotechnology, Vol. 24, No. 7, 07.2008, p. 917-925.

Research output: Contribution to journalArticle

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