Process optimization for the production of sugar for the bioethanol industry from sorghum, a non-conventional source of starch

NK Aggarwal, Poonam Singh - Nee Nigam, D Singh, BS Yadav

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19 Citations (Scopus)

Abstract

A commercial preparation of alpha -amylase, Biotempase, obtained from Biocon India Pvt. Ltd., and crude glucoamylase produced from Aspergillus sp. NA21 were used to hydrolyse sorghum powder, a non-conventional starchy substrate. Among various concentrations of starch (15-35%, dry weight/volume) tried for maximum liquefaction; slurry made with 25% substrate concentration proved optimal. An economical process of liquefaction was carried out using steam under pressure (0.2-0.3 bar, 104-105 degreesC) to liquefy a 25% slurry in just 45 min, contrary to a slower process carried out at 95 degreesC in a water bath. For liquefaction of starch a pH of 5.0 proved to be optimum. The dose of Biotempase as prescribed by the supplier could be reduced by 33% achieving the same degree of liquefaction, by addition of CaCl2 to the starch slurry at the concentration of 200 mg/l. The conditions for the saccharification of liquefied starch were optimized to be 45 degreesC and pH 5.0, producing 90% saccharification in 24 h. Supplementation of divalent ions Ca2+, Mg2+ and Zn2+ in the process of saccharification showed no effect. Finally glucose was found to be the main hydrolysis product in the saccharification of sorghum starch.
LanguageEnglish
Pages411-415
JournalWorld Journal of Microbiology and Biotechnology
Volume17
Issue number4
Publication statusPublished - Jun 2001

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bioethanol
saccharification
starch
industry
sugars
glucan 1,4-alpha-glucosidase
alpha-amylase
Aspergillus
steam
powders
hydrolysis
ions
calcium
India
glucose
liquefaction
dosage
water

Cite this

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title = "Process optimization for the production of sugar for the bioethanol industry from sorghum, a non-conventional source of starch",
abstract = "A commercial preparation of alpha -amylase, Biotempase, obtained from Biocon India Pvt. Ltd., and crude glucoamylase produced from Aspergillus sp. NA21 were used to hydrolyse sorghum powder, a non-conventional starchy substrate. Among various concentrations of starch (15-35{\%}, dry weight/volume) tried for maximum liquefaction; slurry made with 25{\%} substrate concentration proved optimal. An economical process of liquefaction was carried out using steam under pressure (0.2-0.3 bar, 104-105 degreesC) to liquefy a 25{\%} slurry in just 45 min, contrary to a slower process carried out at 95 degreesC in a water bath. For liquefaction of starch a pH of 5.0 proved to be optimum. The dose of Biotempase as prescribed by the supplier could be reduced by 33{\%} achieving the same degree of liquefaction, by addition of CaCl2 to the starch slurry at the concentration of 200 mg/l. The conditions for the saccharification of liquefied starch were optimized to be 45 degreesC and pH 5.0, producing 90{\%} saccharification in 24 h. Supplementation of divalent ions Ca2+, Mg2+ and Zn2+ in the process of saccharification showed no effect. Finally glucose was found to be the main hydrolysis product in the saccharification of sorghum starch.",
author = "NK Aggarwal and {Singh - Nee Nigam}, Poonam and D Singh and BS Yadav",
year = "2001",
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language = "English",
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journal = "World Journal of Microbiology and Biotechnology",
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TY - JOUR

T1 - Process optimization for the production of sugar for the bioethanol industry from sorghum, a non-conventional source of starch

AU - Aggarwal, NK

AU - Singh - Nee Nigam, Poonam

AU - Singh, D

AU - Yadav, BS

PY - 2001/6

Y1 - 2001/6

N2 - A commercial preparation of alpha -amylase, Biotempase, obtained from Biocon India Pvt. Ltd., and crude glucoamylase produced from Aspergillus sp. NA21 were used to hydrolyse sorghum powder, a non-conventional starchy substrate. Among various concentrations of starch (15-35%, dry weight/volume) tried for maximum liquefaction; slurry made with 25% substrate concentration proved optimal. An economical process of liquefaction was carried out using steam under pressure (0.2-0.3 bar, 104-105 degreesC) to liquefy a 25% slurry in just 45 min, contrary to a slower process carried out at 95 degreesC in a water bath. For liquefaction of starch a pH of 5.0 proved to be optimum. The dose of Biotempase as prescribed by the supplier could be reduced by 33% achieving the same degree of liquefaction, by addition of CaCl2 to the starch slurry at the concentration of 200 mg/l. The conditions for the saccharification of liquefied starch were optimized to be 45 degreesC and pH 5.0, producing 90% saccharification in 24 h. Supplementation of divalent ions Ca2+, Mg2+ and Zn2+ in the process of saccharification showed no effect. Finally glucose was found to be the main hydrolysis product in the saccharification of sorghum starch.

AB - A commercial preparation of alpha -amylase, Biotempase, obtained from Biocon India Pvt. Ltd., and crude glucoamylase produced from Aspergillus sp. NA21 were used to hydrolyse sorghum powder, a non-conventional starchy substrate. Among various concentrations of starch (15-35%, dry weight/volume) tried for maximum liquefaction; slurry made with 25% substrate concentration proved optimal. An economical process of liquefaction was carried out using steam under pressure (0.2-0.3 bar, 104-105 degreesC) to liquefy a 25% slurry in just 45 min, contrary to a slower process carried out at 95 degreesC in a water bath. For liquefaction of starch a pH of 5.0 proved to be optimum. The dose of Biotempase as prescribed by the supplier could be reduced by 33% achieving the same degree of liquefaction, by addition of CaCl2 to the starch slurry at the concentration of 200 mg/l. The conditions for the saccharification of liquefied starch were optimized to be 45 degreesC and pH 5.0, producing 90% saccharification in 24 h. Supplementation of divalent ions Ca2+, Mg2+ and Zn2+ in the process of saccharification showed no effect. Finally glucose was found to be the main hydrolysis product in the saccharification of sorghum starch.

M3 - Article

VL - 17

SP - 411

EP - 415

JO - World Journal of Microbiology and Biotechnology

T2 - World Journal of Microbiology and Biotechnology

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