Primary Structure of Neuromedin U from the Rat

J. M. Conlon; J. Domin; L. Thim; V. DiMarzo; H. R. Morris; S. R. Bloom; L. Thim; V. DiMarzo; H. R. Morris

doi:10.1111/j.1471-4159.1988.tb01837.x

Primary Structure of Neuromedin U from the Rat

J. M. Conlon, J. Domin, L. Thim, V. DiMarzo, H. R. Morris, S. R. Bloom, L. Thim, V. DiMarzo, H. R. Morris

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

Abstract: The primary structure of neuromedin U from the rat ileum was established as: Tyr‐Lys‐Val‐Asn‐Glu⁵‐Tyr‐Gln‐Gly‐Pro‐Val¹⁰‐Ala‐Pro‐Ser‐Gly‐Gly¹⁵‐Phe‐Phe‐Leu‐Phe‐Arg²⁰‐Pro‐Arg‐Asn‐NH₂. There was no evidence for microheterogeneity. This amino acid sequence contains two deletions and nine substitutions compared with the neuromedin U‐25 from the pig. In particular, the replacement of the Arg'⁶‐Arg¹⁷ processing site in the porcine peptide by Gly¹⁴‐Gly¹⁵ in the rat means that a peptide corresponding to neuromedin U‐8 was not found in the rat intestine.

Original language	English
Pages (from-to)	988-991
Number of pages	4
Journal	Journal of Neurochemistry
Volume	51
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-4159.1988.tb01837.x
Publication status	Published (in print/issue) - Sept 1988

Keywords

Edman degradation
Neuromedin U
Radioimmunoassay
Rat gastrointestinal tract

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10.1111/j.1471-4159.1988.tb01837.x

Cite this

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title = "Primary Structure of Neuromedin U from the Rat",

abstract = "Abstract: The primary structure of neuromedin U from the rat ileum was established as: Tyr‐Lys‐Val‐Asn‐Glu5‐Tyr‐Gln‐Gly‐Pro‐Val10‐Ala‐Pro‐Ser‐Gly‐Gly15‐Phe‐Phe‐Leu‐Phe‐Arg20‐Pro‐Arg‐Asn‐NH2. There was no evidence for microheterogeneity. This amino acid sequence contains two deletions and nine substitutions compared with the neuromedin U‐25 from the pig. In particular, the replacement of the Arg'6‐Arg17 processing site in the porcine peptide by Gly14‐Gly15 in the rat means that a peptide corresponding to neuromedin U‐8 was not found in the rat intestine.",

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author = "Conlon, {J. M.} and J. Domin and L. Thim and V. DiMarzo and Morris, {H. R.} and Bloom, {S. R.} and L. Thim and V. DiMarzo and Morris, {H. R.}",

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T1 - Primary Structure of Neuromedin U from the Rat

AU - Conlon, J. M.

AU - Domin, J.

AU - Thim, L.

AU - DiMarzo, V.

AU - Morris, H. R.

AU - Bloom, S. R.

AU - Thim, L.

AU - DiMarzo, V.

AU - Morris, H. R.

PY - 1988/9

Y1 - 1988/9

N2 - Abstract: The primary structure of neuromedin U from the rat ileum was established as: Tyr‐Lys‐Val‐Asn‐Glu5‐Tyr‐Gln‐Gly‐Pro‐Val10‐Ala‐Pro‐Ser‐Gly‐Gly15‐Phe‐Phe‐Leu‐Phe‐Arg20‐Pro‐Arg‐Asn‐NH2. There was no evidence for microheterogeneity. This amino acid sequence contains two deletions and nine substitutions compared with the neuromedin U‐25 from the pig. In particular, the replacement of the Arg'6‐Arg17 processing site in the porcine peptide by Gly14‐Gly15 in the rat means that a peptide corresponding to neuromedin U‐8 was not found in the rat intestine.

AB - Abstract: The primary structure of neuromedin U from the rat ileum was established as: Tyr‐Lys‐Val‐Asn‐Glu5‐Tyr‐Gln‐Gly‐Pro‐Val10‐Ala‐Pro‐Ser‐Gly‐Gly15‐Phe‐Phe‐Leu‐Phe‐Arg20‐Pro‐Arg‐Asn‐NH2. There was no evidence for microheterogeneity. This amino acid sequence contains two deletions and nine substitutions compared with the neuromedin U‐25 from the pig. In particular, the replacement of the Arg'6‐Arg17 processing site in the porcine peptide by Gly14‐Gly15 in the rat means that a peptide corresponding to neuromedin U‐8 was not found in the rat intestine.

KW - Edman degradation

KW - Neuromedin U

KW - Radioimmunoassay

KW - Rat gastrointestinal tract

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DO - 10.1111/j.1471-4159.1988.tb01837.x

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SP - 988

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JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

IS - 3

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Primary Structure of Neuromedin U from the Rat

Abstract

Keywords

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