Primary structure of insulin and glucagon from the flounder (Platichthys flesus)

J. Michael Conlon; Michael S. Davis; Lars Thim

doi:10.1016/0016-6480(87)90268-1

Primary structure of insulin and glucagon from the flounder (Platichthys flesus)

J. Michael Conlon
, Michael S. Davis
, Lars Thim

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Insulin and glucagon have been isolated from the Brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated Edman degradation. The A-chain of flounder insulin shows strong homology to the A-chains from the coho salmon (Oncorhynchus kisutch; 100%) and the anglerfish (Lophius americanus; 95%) but homologies in the B-chain region are weaker (salmon 79%, anglerfish 83%). Flounder insulin B-chain contains the novel sequence Val-Val-Pro-Pro at the NH₂ terminus and the highly conserved seryl residue at position 10 (B 9 in mammals) is replaced by an alanyl residue. Flounder glucagon is identical to anglerfish glucagon II but shows four amino acid substitutions compared with salmon glucagon.

Original language	English
Pages (from-to)	203-209
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/0016-6480(87)90268-1
Publication status	Published (in print/issue) - May 1987

Funding

This work was supported by the Stiftung Vofks-wagenwerk and SERC (UK) Grant GRID 18097. The authors thank Professor N. Hilschmann, Max-Planck-Institut fiir Experimentelle Medizin, Giittingen, for providing facilities for amino acid analysis.

Funder number
GRID 18097

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10.1016/0016-6480(87)90268-1

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abstract = "Insulin and glucagon have been isolated from the Brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated Edman degradation. The A-chain of flounder insulin shows strong homology to the A-chains from the coho salmon (Oncorhynchus kisutch; 100\%) and the anglerfish (Lophius americanus; 95\%) but homologies in the B-chain region are weaker (salmon 79\%, anglerfish 83\%). Flounder insulin B-chain contains the novel sequence Val-Val-Pro-Pro at the NH2 terminus and the highly conserved seryl residue at position 10 (B 9 in mammals) is replaced by an alanyl residue. Flounder glucagon is identical to anglerfish glucagon II but shows four amino acid substitutions compared with salmon glucagon.",

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AU - Conlon, J. Michael

AU - Davis, Michael S.

AU - Thim, Lars

PY - 1987/5

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N2 - Insulin and glucagon have been isolated from the Brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated Edman degradation. The A-chain of flounder insulin shows strong homology to the A-chains from the coho salmon (Oncorhynchus kisutch; 100%) and the anglerfish (Lophius americanus; 95%) but homologies in the B-chain region are weaker (salmon 79%, anglerfish 83%). Flounder insulin B-chain contains the novel sequence Val-Val-Pro-Pro at the NH2 terminus and the highly conserved seryl residue at position 10 (B 9 in mammals) is replaced by an alanyl residue. Flounder glucagon is identical to anglerfish glucagon II but shows four amino acid substitutions compared with salmon glucagon.

AB - Insulin and glucagon have been isolated from the Brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated Edman degradation. The A-chain of flounder insulin shows strong homology to the A-chains from the coho salmon (Oncorhynchus kisutch; 100%) and the anglerfish (Lophius americanus; 95%) but homologies in the B-chain region are weaker (salmon 79%, anglerfish 83%). Flounder insulin B-chain contains the novel sequence Val-Val-Pro-Pro at the NH2 terminus and the highly conserved seryl residue at position 10 (B 9 in mammals) is replaced by an alanyl residue. Flounder glucagon is identical to anglerfish glucagon II but shows four amino acid substitutions compared with salmon glucagon.

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Primary structure of insulin and glucagon from the flounder (Platichthys flesus)

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