Primary structure of frog PYY: Implications for the molecular evolution of the pancreatic polypeptide family

A peptide belonging to the pancreatic polypeptide (PP) family was isolated in pure form from the intestine of the European green frog (Rana ridibunda). The primary structure of the peptide was established as: Tyr-Pro-Pro-Lys-Pro-Glu-Asn-Pro-Gly-Glu¹⁰-Asp-Ala-Ser-Pro-Glu-Glu-Met-Thr-Lys-Tyr²⁰-Leu-Thr- Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu³⁰-Val-Thr-Arg-Gln-Arg-Tyr-NH₂. This amino acid sequence shows moderate structural similarity to human PYY (75% identity) but stronger similarity to the PP family peptides isolated from the pancreas of the salmon (86%) and dogfish (83%). The data suggest that the two putative duplications of an ancestral PP family gene that have given rise to PP, PYY and NPY in mammals had already taken place by the time of the appearance of the amphibia. In fish, however, only a single duplication has occurred, giving rise to NPY in nervous tissue and a PYY-related peptide in both pancreas and gut.