Prevalence of KIR B haplotype in Irish rheumatoid arthritis patients

Cathy McGeough, David Gibson, Mary Slevin, Gary Wright, Philip Gardiner, Oliver Fitzgerald, AJ Bjourson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Background: Killer cell immunoglobulin like receptors (KIR) expressed on NK and T cell subsets interact with HLA ligands to influence the reactivity of these cells. The KIR/HLA genotype of an individual can affect immune responses to infection and susceptibility to autoimmunity based on the number/type of genes present (1-3).Objectives: The aim of this study was to evaluate KIR/HLA-C genotypes and haplotypes in rheumatoid arthritis patients and healthy controls.Methods: One hundred and fifty one rheumatoid arthritis (RA) patients and 100 healthy controls (HC) were DNA typed for 15 KIR genes and HLA-C group 1 and 2 alleles by PCR-SSP. KIR gene content in each group was determined by direct counting and broad haplotypes AA and Bx were assigned. The Bx haplotype was defined by the presence of inhibiting KIR 2DL2, 2DL5 and/or activating KIR 2DS1, 2DS2, 2DS3, 2DS5 or 3DS1. In the AA haplotype all these genes are absent.Results: Comparison of individual KIR gene content between the RA group and controls did not show any significant differences. The availability of HLA-C1 and C2 ligands for inhibitory KIR was also similar between the RA and HC groups, C1/C1 (50%), C2/C2 (10%), C1/C2 (40%). Analysis of KIR genotypes and haplotype assignation revealed that the Bx haplotype which contains more than one activating KIR was significantly more frequent in the RA group compared to controls (76% vs. 60 %; P = 0.01).Conclusions: Prevalence of the Bx haplotype in this RA population and subsequent availability of more activating KIR on NK and T cells could promote inflammatory responses in this disease.
LanguageEnglish
Title of host publicationUnknown Host Publication
PagesA786
Number of pages1
Volume72
DOIs
Publication statusPublished - 2013
EventEuropean League Against Rheumatism - Madrid, Spain
Duration: 1 Jan 2013 → …

Conference

ConferenceEuropean League Against Rheumatism
Period1/01/13 → …

Fingerprint

Haplotypes
Rheumatoid Arthritis
HLA-C Antigens
Genes
Genotype
Natural Killer Cells
KIR Receptors
Ligands
Control Groups
T-Lymphocyte Subsets
Autoimmunity
Alleles
T-Lymphocytes
Polymerase Chain Reaction
DNA
Infection
Population

Keywords

  • Genetics
  • rheumatoid arthritis

Cite this

McGeough, Cathy ; Gibson, David ; Slevin, Mary ; Wright, Gary ; Gardiner, Philip ; Fitzgerald, Oliver ; Bjourson, AJ. / Prevalence of KIR B haplotype in Irish rheumatoid arthritis patients. Unknown Host Publication. Vol. 72 2013. pp. A786
@inproceedings{6d903dc3896b4695a9802ffdbee1854d,
title = "Prevalence of KIR B haplotype in Irish rheumatoid arthritis patients",
abstract = "Background: Killer cell immunoglobulin like receptors (KIR) expressed on NK and T cell subsets interact with HLA ligands to influence the reactivity of these cells. The KIR/HLA genotype of an individual can affect immune responses to infection and susceptibility to autoimmunity based on the number/type of genes present (1-3).Objectives: The aim of this study was to evaluate KIR/HLA-C genotypes and haplotypes in rheumatoid arthritis patients and healthy controls.Methods: One hundred and fifty one rheumatoid arthritis (RA) patients and 100 healthy controls (HC) were DNA typed for 15 KIR genes and HLA-C group 1 and 2 alleles by PCR-SSP. KIR gene content in each group was determined by direct counting and broad haplotypes AA and Bx were assigned. The Bx haplotype was defined by the presence of inhibiting KIR 2DL2, 2DL5 and/or activating KIR 2DS1, 2DS2, 2DS3, 2DS5 or 3DS1. In the AA haplotype all these genes are absent.Results: Comparison of individual KIR gene content between the RA group and controls did not show any significant differences. The availability of HLA-C1 and C2 ligands for inhibitory KIR was also similar between the RA and HC groups, C1/C1 (50{\%}), C2/C2 (10{\%}), C1/C2 (40{\%}). Analysis of KIR genotypes and haplotype assignation revealed that the Bx haplotype which contains more than one activating KIR was significantly more frequent in the RA group compared to controls (76{\%} vs. 60 {\%}; P = 0.01).Conclusions: Prevalence of the Bx haplotype in this RA population and subsequent availability of more activating KIR on NK and T cells could promote inflammatory responses in this disease.",
keywords = "Genetics, rheumatoid arthritis",
author = "Cathy McGeough and David Gibson and Mary Slevin and Gary Wright and Philip Gardiner and Oliver Fitzgerald and AJ Bjourson",
year = "2013",
doi = "10.1136/annrheumdis-2013-eular.2324",
language = "English",
volume = "72",
pages = "A786",
booktitle = "Unknown Host Publication",

}

McGeough, C, Gibson, D, Slevin, M, Wright, G, Gardiner, P, Fitzgerald, O & Bjourson, AJ 2013, Prevalence of KIR B haplotype in Irish rheumatoid arthritis patients. in Unknown Host Publication. vol. 72, pp. A786, European League Against Rheumatism, 1/01/13. https://doi.org/10.1136/annrheumdis-2013-eular.2324

Prevalence of KIR B haplotype in Irish rheumatoid arthritis patients. / McGeough, Cathy; Gibson, David; Slevin, Mary; Wright, Gary; Gardiner, Philip; Fitzgerald, Oliver; Bjourson, AJ.

Unknown Host Publication. Vol. 72 2013. p. A786.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Prevalence of KIR B haplotype in Irish rheumatoid arthritis patients

AU - McGeough, Cathy

AU - Gibson, David

AU - Slevin, Mary

AU - Wright, Gary

AU - Gardiner, Philip

AU - Fitzgerald, Oliver

AU - Bjourson, AJ

PY - 2013

Y1 - 2013

N2 - Background: Killer cell immunoglobulin like receptors (KIR) expressed on NK and T cell subsets interact with HLA ligands to influence the reactivity of these cells. The KIR/HLA genotype of an individual can affect immune responses to infection and susceptibility to autoimmunity based on the number/type of genes present (1-3).Objectives: The aim of this study was to evaluate KIR/HLA-C genotypes and haplotypes in rheumatoid arthritis patients and healthy controls.Methods: One hundred and fifty one rheumatoid arthritis (RA) patients and 100 healthy controls (HC) were DNA typed for 15 KIR genes and HLA-C group 1 and 2 alleles by PCR-SSP. KIR gene content in each group was determined by direct counting and broad haplotypes AA and Bx were assigned. The Bx haplotype was defined by the presence of inhibiting KIR 2DL2, 2DL5 and/or activating KIR 2DS1, 2DS2, 2DS3, 2DS5 or 3DS1. In the AA haplotype all these genes are absent.Results: Comparison of individual KIR gene content between the RA group and controls did not show any significant differences. The availability of HLA-C1 and C2 ligands for inhibitory KIR was also similar between the RA and HC groups, C1/C1 (50%), C2/C2 (10%), C1/C2 (40%). Analysis of KIR genotypes and haplotype assignation revealed that the Bx haplotype which contains more than one activating KIR was significantly more frequent in the RA group compared to controls (76% vs. 60 %; P = 0.01).Conclusions: Prevalence of the Bx haplotype in this RA population and subsequent availability of more activating KIR on NK and T cells could promote inflammatory responses in this disease.

AB - Background: Killer cell immunoglobulin like receptors (KIR) expressed on NK and T cell subsets interact with HLA ligands to influence the reactivity of these cells. The KIR/HLA genotype of an individual can affect immune responses to infection and susceptibility to autoimmunity based on the number/type of genes present (1-3).Objectives: The aim of this study was to evaluate KIR/HLA-C genotypes and haplotypes in rheumatoid arthritis patients and healthy controls.Methods: One hundred and fifty one rheumatoid arthritis (RA) patients and 100 healthy controls (HC) were DNA typed for 15 KIR genes and HLA-C group 1 and 2 alleles by PCR-SSP. KIR gene content in each group was determined by direct counting and broad haplotypes AA and Bx were assigned. The Bx haplotype was defined by the presence of inhibiting KIR 2DL2, 2DL5 and/or activating KIR 2DS1, 2DS2, 2DS3, 2DS5 or 3DS1. In the AA haplotype all these genes are absent.Results: Comparison of individual KIR gene content between the RA group and controls did not show any significant differences. The availability of HLA-C1 and C2 ligands for inhibitory KIR was also similar between the RA and HC groups, C1/C1 (50%), C2/C2 (10%), C1/C2 (40%). Analysis of KIR genotypes and haplotype assignation revealed that the Bx haplotype which contains more than one activating KIR was significantly more frequent in the RA group compared to controls (76% vs. 60 %; P = 0.01).Conclusions: Prevalence of the Bx haplotype in this RA population and subsequent availability of more activating KIR on NK and T cells could promote inflammatory responses in this disease.

KW - Genetics

KW - rheumatoid arthritis

U2 - 10.1136/annrheumdis-2013-eular.2324

DO - 10.1136/annrheumdis-2013-eular.2324

M3 - Conference contribution

VL - 72

SP - A786

BT - Unknown Host Publication

ER -