Preparation of ultrathin grafts for Descemet-stripping endothelial keratoplasty with a single microkeratome pass

Vito Romano, Bernhard Steger, Jayavani Myneni, Mark Batterbury, Colin Willoughby, Stephen B. Kaye

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    We present a technique to achieve ultrathin Descemet-stripping automated endothelial keratoplasty (DSAEK). Using a simple method of controlling artificial anterior chamber pressure and drying the corneal surface, it was possible to thin the donor cornea at a rate of 11 μm a minute. When the donor cornea was between 500 μm and 510 μm, a single pass was made using a 350 μm microkeratome head followed by a peripheral dissection. The resulting mean graft thickness was 83.2 μm ± 14.9 (SD) (range 50 to 98 μm) with a mean peripheral graft edge thickness of 106.8 ± 10.9 μm (range 90 to 120 μm). There were no surgical complications, and all grafts remained attached. This is a reliable method for preparing ultrathin donor corneal lenticules for DSAEK in the operating room or eye bank without using multiple microkeratome heads or risking double passes.
    Original languageEnglish
    Pages (from-to)12-15
    JournalJournal of Cataract & Refractive Surgery
    Issue number1
    Publication statusAccepted/In press - 31 Aug 2016



    • cornea
    • endothelium
    • Descemet-stripping automated endothelial keratoplasty

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