Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

Natalie Finch; Benita Percival; Elena Hunter; Robin J. Blagg; Emily Blackwell; James Sagar; Zeeshan Ahmad; Ming-Wei Chang; John A. Hunt; Melissa L. Mather; Séverine Tasker; Luisa De Risio; Philippe B. Wilson

doi:10.1186/s13104-021-05888-y

Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

Natalie Finch, Benita Percival, Elena Hunter, Robin J. Blagg, Emily Blackwell, James Sagar, Zeeshan Ahmad, Ming-Wei Chang, John A. Hunt, Melissa L. Mather, Séverine Tasker, Luisa De Risio, Philippe B. Wilson

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Abstract

Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making.
Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

Original language	English
Article number	469
Pages (from-to)	1-5
Number of pages	5
Journal	BMC Research Notes
Volume	14
Issue number	1
Early online date	24 Dec 2021
DOIs	https://doi.org/10.1186/s13104-021-05888-y
Publication status	Published online - 24 Dec 2021

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

Keywords

Research Note
Chronic kidney disease
Cat
Metabolite
Metabolomics
NMR

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Finch, N., Percival, B., Hunter, E., Blagg, R. J., Blackwell, E., Sagar, J., Ahmad, Z., Chang, M.-W., Hunt, J. A., Mather, M. L., Tasker, S., De Risio, L., & Wilson, P. B. (2021). Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study. BMC Research Notes, 14(1), 1-5. Article 469. Advance online publication. https://doi.org/10.1186/s13104-021-05888-y

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abstract = "Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making. Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.",

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doi = "10.1186/s13104-021-05888-y",

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AU - Blackwell, Emily

AU - Sagar, James

AU - Ahmad, Zeeshan

AU - Chang, Ming-Wei

AU - Hunt, John A.

AU - Mather, Melissa L.

AU - Tasker, Séverine

AU - De Risio, Luisa

AU - Wilson, Philippe B.

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AB - Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making. Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

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Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

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