Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype.

Nor F Rajab, Declan McKenna, Jim Diamond, Kate Williamson, Peter W Hamilton, VJ McKelvey-Martin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

BACKGROUNDNuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation.METHODSThe alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to gamma-radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity.RESULTSComet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75% of all cells into their radiosensitive or radioresistant groups based on their pretreatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation.CONCLUSIONSThe authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy.
LanguageEnglish
Pages1077-85
JournalCytometry Part A
Volume69
Issue number10
Publication statusPublished - 2006

Fingerprint

Radiation Tolerance
Chromatin
Urinary Bladder
Phenotype
Cell Line
Comet Assay
Gamma Rays
Discriminant Analysis
Ionizing Radiation
Cell Nucleus
Radiotherapy
Radiation
Carcinoma
DNA

Keywords

  • nuclear texture
  • chromatin morphology
  • radiosensitivity
  • comet assay
  • bladder

Cite this

Rajab, N. F., McKenna, D., Diamond, J., Williamson, K., Hamilton, P. W., & McKelvey-Martin, VJ. (2006). Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. Cytometry Part A, 69(10), 1077-85.
Rajab, Nor F ; McKenna, Declan ; Diamond, Jim ; Williamson, Kate ; Hamilton, Peter W ; McKelvey-Martin, VJ. / Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. In: Cytometry Part A. 2006 ; Vol. 69, No. 10. pp. 1077-85.
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abstract = "BACKGROUNDNuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation.METHODSThe alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to gamma-radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity.RESULTSComet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75{\%} of all cells into their radiosensitive or radioresistant groups based on their pretreatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation.CONCLUSIONSThe authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy.",
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Rajab, NF, McKenna, D, Diamond, J, Williamson, K, Hamilton, PW & McKelvey-Martin, VJ 2006, 'Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype.', Cytometry Part A, vol. 69, no. 10, pp. 1077-85.

Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. / Rajab, Nor F; McKenna, Declan; Diamond, Jim; Williamson, Kate; Hamilton, Peter W; McKelvey-Martin, VJ.

In: Cytometry Part A, Vol. 69, No. 10, 2006, p. 1077-85.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype.

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AU - Williamson, Kate

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AU - McKelvey-Martin, VJ

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N2 - BACKGROUNDNuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation.METHODSThe alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to gamma-radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity.RESULTSComet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75% of all cells into their radiosensitive or radioresistant groups based on their pretreatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation.CONCLUSIONSThe authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy.

AB - BACKGROUNDNuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation.METHODSThe alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to gamma-radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity.RESULTSComet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75% of all cells into their radiosensitive or radioresistant groups based on their pretreatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation.CONCLUSIONSThe authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy.

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Rajab NF, McKenna D, Diamond J, Williamson K, Hamilton PW, McKelvey-Martin VJ. Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. Cytometry Part A. 2006;69(10):1077-85.