Predatory bacteria in combination with solar disinfection and solar photocatalysis for the treatment of rainwater

Monique Waso; S. Khan; Anukriti Singh; Stuart McMichael; W. Ahmed; Pilar Fernandez-Ibanez; John Byrne; Wesaal Khan

doi:10.1016/j.watres.2019.115281

Predatory bacteria in combination with solar disinfection and solar photocatalysis for the treatment of rainwater

Monique Waso
, S. Khan
, Anukriti Singh
, Stuart McMichael
, W. Ahmed
, Pilar Fernandez-Ibanez
, John Byrne
, Wesaal Khan

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

419 Downloads (Pure)

Abstract

The predatory bacterium, Bdellovibrio bacteriovorus, was applied as a biological pre-treatment to solar disinfection and solar photocatalytic disinfection for rainwater treatment. The photocatalyst used was immobilised titanium-dioxide reduced graphene oxide. The pre-treatment followed by solar photocatalysis for 120 min under natural sunlight reduced the viable counts of Klebsiella pneumoniae from 2.00 × 10 ⁹ colony forming units (CFU)/mL to below the detection limit (BDL) (<1 CFU/100 μL). Correspondingly, ethidium monoazide bromide quantitative PCR analysis indicated a high total log reduction in K. pneumoniae gene copies (GC)/mL (5.85 logs after solar photocatalysis for 240 min). In contrast, solar disinfection and solar photocatalysis without the biological pre-treatment were more effective for Enterococcus faecium disinfection as the viable counts of E. faecium were reduced by 8.00 logs (from 1.00 × 10 ⁸ CFU/mL to BDL) and the gene copies were reduced by ∼3.39 logs (from 2.09 × 10 ⁶ GC/mL to ∼9.00 × 10 ² GC/mL) after 240 min of treatment. Predatory bacteria can be applied as a pre-treatment to solar disinfection and solar photocatalytic treatment to enhance the removal efficiency of Gram-negative bacteria, which is crucial for the development of a targeted water treatment approach.

Original language	English
Article number	115281
Pages (from-to)	1
Number of pages	9
Journal	Water Research
Volume	169
Issue number	1
Early online date	8 Nov 2019
DOIs	https://doi.org/10.1016/j.watres.2019.115281
Publication status	Published (in print/issue) - 1 Feb 2020

Bibliographical note

Funding Information:
The authors would like to acknowledge the following individuals and institutions for their contribution to this project: • The financial assistance of the Deutscher Akademischer Austauschdienst / National Research Foundation of South Africa and the Royal Society Newton Mobility Grant (Grant number: NI170184 ). Opinions expressed and conclusions arrived at, are those of the authors and are not necessarily to be attributed to the DAAD/NRF or the Royal Society Newton Mobility Grant. • Pilar Fernández-Ibáñez and John Anthony Byrne acknowledge funding from Global Challenges Research Fund – United Kingdom Research and Innovation (GCRF UKRI) for SAFEWATER (Grant Ref number EP/P032427/1 ). • Dr Jeremy Hamilton and Dr Preetam Sharma from the Nanotechnology and Integrated BioEngineering Centre (NIBEC) at Ulster University (Northern Ireland) for their guidance during the synthesis and immobilisation of the TiO 2 -rGO material. • Casper Brink from the Microbiology Department at Stellenbosch University for creating the drawings of the designed SODIS systems in AutoCAD® 2018. • Dr Pieter Neethling from the Physics Department at Stellenbosch University for measuring the reflectance of the stainless-steel sheets utilised for the construction of the compound parabolic collectors. Appendix A

Publisher Copyright:
© 2019 Elsevier Ltd

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Funding

Funding Information: The authors would like to acknowledge the following individuals and institutions for their contribution to this project: • The financial assistance of the Deutscher Akademischer Austauschdienst / National Research Foundation of South Africa and the Royal Society Newton Mobility Grant (Grant number: NI170184 ). Opinions expressed and conclusions arrived at, are those of the authors and are not necessarily to be attributed to the DAAD/NRF or the Royal Society Newton Mobility Grant. • Pilar Fernández-Ibáñez and John Anthony Byrne acknowledge funding from Global Challenges Research Fund – United Kingdom Research and Innovation (GCRF UKRI) for SAFEWATER (Grant Ref number EP/P032427/1 ). • Dr Jeremy Hamilton and Dr Preetam Sharma from the Nanotechnology and Integrated BioEngineering Centre (NIBEC) at Ulster University (Northern Ireland) for their guidance during the synthesis and immobilisation of the TiO 2 -rGO material. • Casper Brink from the Microbiology Department at Stellenbosch University for creating the drawings of the designed SODIS systems in AutoCAD® 2018. • Dr Pieter Neethling from the Physics Department at Stellenbosch University for measuring the reflectance of the stainless-steel sheets utilised for the construction of the compound parabolic collectors. Appendix A Publisher Copyright: © 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Keywords

Bdellovibrio bacteriovorus
Biological pre-treatment
Harvested rainwater
Photocatalysis
Solar disinfection

Access to Document

10.1016/j.watres.2019.115281

Predator_SODIS _accepted manuscriptAuthor Accepted Manuscript, 310 KB

Cite this

@article{f27d0f83c7114429a2c18c3bd84fd429,

title = "Predatory bacteria in combination with solar disinfection and solar photocatalysis for the treatment of rainwater",

abstract = "The predatory bacterium, Bdellovibrio bacteriovorus, was applied as a biological pre-treatment to solar disinfection and solar photocatalytic disinfection for rainwater treatment. The photocatalyst used was immobilised titanium-dioxide reduced graphene oxide. The pre-treatment followed by solar photocatalysis for 120 min under natural sunlight reduced the viable counts of Klebsiella pneumoniae from 2.00 × 10 9 colony forming units (CFU)/mL to below the detection limit (BDL) (<1 CFU/100 μL). Correspondingly, ethidium monoazide bromide quantitative PCR analysis indicated a high total log reduction in K. pneumoniae gene copies (GC)/mL (5.85 logs after solar photocatalysis for 240 min). In contrast, solar disinfection and solar photocatalysis without the biological pre-treatment were more effective for Enterococcus faecium disinfection as the viable counts of E. faecium were reduced by 8.00 logs (from 1.00 × 10 8 CFU/mL to BDL) and the gene copies were reduced by ∼3.39 logs (from 2.09 × 10 6 GC/mL to ∼9.00 × 10 2 GC/mL) after 240 min of treatment. Predatory bacteria can be applied as a pre-treatment to solar disinfection and solar photocatalytic treatment to enhance the removal efficiency of Gram-negative bacteria, which is crucial for the development of a targeted water treatment approach. ",

keywords = "Bdellovibrio bacteriovorus, Biological pre-treatment, Harvested rainwater, Photocatalysis, Solar disinfection",

author = "Monique Waso and S. Khan and Anukriti Singh and Stuart McMichael and W. Ahmed and Pilar Fernandez-Ibanez and John Byrne and Wesaal Khan",

note = "Funding Information: The authors would like to acknowledge the following individuals and institutions for their contribution to this project: • The financial assistance of the Deutscher Akademischer Austauschdienst / National Research Foundation of South Africa and the Royal Society Newton Mobility Grant (Grant number: NI170184 ). Opinions expressed and conclusions arrived at, are those of the authors and are not necessarily to be attributed to the DAAD/NRF or the Royal Society Newton Mobility Grant. • Pilar Fern{\'a}ndez-Ib{\'a}{\~n}ez and John Anthony Byrne acknowledge funding from Global Challenges Research Fund – United Kingdom Research and Innovation (GCRF UKRI) for SAFEWATER (Grant Ref number EP/P032427/1 ). • Dr Jeremy Hamilton and Dr Preetam Sharma from the Nanotechnology and Integrated BioEngineering Centre (NIBEC) at Ulster University (Northern Ireland) for their guidance during the synthesis and immobilisation of the TiO 2 -rGO material. • Casper Brink from the Microbiology Department at Stellenbosch University for creating the drawings of the designed SODIS systems in AutoCAD{\textregistered} 2018. • Dr Pieter Neethling from the Physics Department at Stellenbosch University for measuring the reflectance of the stainless-steel sheets utilised for the construction of the compound parabolic collectors. Appendix A Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = feb,

day = "1",

doi = "10.1016/j.watres.2019.115281",

language = "English",

volume = "169",

pages = "1",

journal = "Water Research",

issn = "0043-1354",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Predatory bacteria in combination with solar disinfection and solar photocatalysis for the treatment of rainwater

AU - Waso, Monique

AU - Khan, S.

AU - Singh, Anukriti

AU - McMichael, Stuart

AU - Ahmed, W.

AU - Fernandez-Ibanez, Pilar

AU - Byrne, John

AU - Khan, Wesaal

N1 - Funding Information: The authors would like to acknowledge the following individuals and institutions for their contribution to this project: • The financial assistance of the Deutscher Akademischer Austauschdienst / National Research Foundation of South Africa and the Royal Society Newton Mobility Grant (Grant number: NI170184 ). Opinions expressed and conclusions arrived at, are those of the authors and are not necessarily to be attributed to the DAAD/NRF or the Royal Society Newton Mobility Grant. • Pilar Fernández-Ibáñez and John Anthony Byrne acknowledge funding from Global Challenges Research Fund – United Kingdom Research and Innovation (GCRF UKRI) for SAFEWATER (Grant Ref number EP/P032427/1 ). • Dr Jeremy Hamilton and Dr Preetam Sharma from the Nanotechnology and Integrated BioEngineering Centre (NIBEC) at Ulster University (Northern Ireland) for their guidance during the synthesis and immobilisation of the TiO 2 -rGO material. • Casper Brink from the Microbiology Department at Stellenbosch University for creating the drawings of the designed SODIS systems in AutoCAD® 2018. • Dr Pieter Neethling from the Physics Department at Stellenbosch University for measuring the reflectance of the stainless-steel sheets utilised for the construction of the compound parabolic collectors. Appendix A Publisher Copyright: © 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/2/1

Y1 - 2020/2/1

N2 - The predatory bacterium, Bdellovibrio bacteriovorus, was applied as a biological pre-treatment to solar disinfection and solar photocatalytic disinfection for rainwater treatment. The photocatalyst used was immobilised titanium-dioxide reduced graphene oxide. The pre-treatment followed by solar photocatalysis for 120 min under natural sunlight reduced the viable counts of Klebsiella pneumoniae from 2.00 × 10 9 colony forming units (CFU)/mL to below the detection limit (BDL) (<1 CFU/100 μL). Correspondingly, ethidium monoazide bromide quantitative PCR analysis indicated a high total log reduction in K. pneumoniae gene copies (GC)/mL (5.85 logs after solar photocatalysis for 240 min). In contrast, solar disinfection and solar photocatalysis without the biological pre-treatment were more effective for Enterococcus faecium disinfection as the viable counts of E. faecium were reduced by 8.00 logs (from 1.00 × 10 8 CFU/mL to BDL) and the gene copies were reduced by ∼3.39 logs (from 2.09 × 10 6 GC/mL to ∼9.00 × 10 2 GC/mL) after 240 min of treatment. Predatory bacteria can be applied as a pre-treatment to solar disinfection and solar photocatalytic treatment to enhance the removal efficiency of Gram-negative bacteria, which is crucial for the development of a targeted water treatment approach.

AB - The predatory bacterium, Bdellovibrio bacteriovorus, was applied as a biological pre-treatment to solar disinfection and solar photocatalytic disinfection for rainwater treatment. The photocatalyst used was immobilised titanium-dioxide reduced graphene oxide. The pre-treatment followed by solar photocatalysis for 120 min under natural sunlight reduced the viable counts of Klebsiella pneumoniae from 2.00 × 10 9 colony forming units (CFU)/mL to below the detection limit (BDL) (<1 CFU/100 μL). Correspondingly, ethidium monoazide bromide quantitative PCR analysis indicated a high total log reduction in K. pneumoniae gene copies (GC)/mL (5.85 logs after solar photocatalysis for 240 min). In contrast, solar disinfection and solar photocatalysis without the biological pre-treatment were more effective for Enterococcus faecium disinfection as the viable counts of E. faecium were reduced by 8.00 logs (from 1.00 × 10 8 CFU/mL to BDL) and the gene copies were reduced by ∼3.39 logs (from 2.09 × 10 6 GC/mL to ∼9.00 × 10 2 GC/mL) after 240 min of treatment. Predatory bacteria can be applied as a pre-treatment to solar disinfection and solar photocatalytic treatment to enhance the removal efficiency of Gram-negative bacteria, which is crucial for the development of a targeted water treatment approach.

KW - Bdellovibrio bacteriovorus

KW - Biological pre-treatment

KW - Harvested rainwater

KW - Photocatalysis

KW - Solar disinfection

UR - https://www.scopus.com/pages/publications/85074777431

U2 - 10.1016/j.watres.2019.115281

DO - 10.1016/j.watres.2019.115281

M3 - Article

C2 - 31733621

SN - 0043-1354

VL - 169

SP - 1

JO - Water Research

JF - Water Research

IS - 1

M1 - 115281

ER -

Predatory bacteria in combination with solar disinfection and solar photocatalysis for the treatment of rainwater

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

2D heterostructured photocatalytic materials for water treatment and disinfection

Cite this

Predatory bacteria in combination with solar disinfection and solar photocatalysis for the treatment of rainwater

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Student theses

2D heterostructured photocatalytic materials for water treatment and disinfection

Cite this