Abstract
| Language | English |
|---|---|
| Journal | Frontiers in bioengineering and biotechnology |
| DOIs | |
| Publication status | Accepted/In press - 6 Nov 2019 |
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Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties. / Mancuso, Elena; Tonda-Turo, Chiara; Ceresa, Chiara; Pensabene, Virginia; Connell, Simon; Fracchia, Letizia; Gentile, Piergiorgio.
In: Frontiers in bioengineering and biotechnology, 06.11.2019.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties
AU - Mancuso, Elena
AU - Tonda-Turo, Chiara
AU - Ceresa, Chiara
AU - Pensabene, Virginia
AU - Connell, Simon
AU - Fracchia, Letizia
AU - Gentile, Piergiorgio
PY - 2019/11/6
Y1 - 2019/11/6
N2 - The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.
AB - The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.
U2 - 10.3389/fbioe.2019.00344
DO - 10.3389/fbioe.2019.00344
M3 - Review article
JO - Frontiers in bioengineering and biotechnology
T2 - Frontiers in bioengineering and biotechnology
JF - Frontiers in bioengineering and biotechnology
SN - 2296-4185
ER -