Potential of Manuka Honey as a Natural Polyelectrolyte to Develop Biomimetic Nanostructured Meshes With Antimicrobial Properties

Elena Mancuso, Chiara Tonda-Turo, Chiara Ceresa, Virginia Pensabene, Simon Connell, Letizia Fracchia, Piergiorgio Gentile

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)
59 Downloads (Pure)

Abstract

The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic-resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects; (2) rediscovering the beneficial effects of naturally derived agents; and (3) preserving the substrate functional properties. For the first time, Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via layer-by-layer assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physicochemical characterization demonstrated the successful nanocoating formation. Different cell lines (human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated that the antimicrobial MH activity was dependent on the concentration used and strains tested.

Original languageEnglish
Article number344
JournalFrontiers in bioengineering and biotechnology
Volume7
DOIs
Publication statusPublished (in print/issue) - 4 Dec 2019

Bibliographical note

This research was supported by the UK NIHR-EPSRC IMPRESS Network (EP/M000109/1 and EP/N027345/1) and by PROM project (748903), funded by H2020-MSCA-IF-2016. Microbiological assays are supported by the Università del Piemonte Orientale (local research project 2016-ex60%).

Keywords

  • Manuka honey
  • electrospinning
  • layer-by-layer assembly
  • manofunctionalization
  • soft tissue regeneration

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