Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties

Elena Mancuso, Chiara Tonda-Turo, Chiara Ceresa, Virginia Pensabene, Simon Connell, Letizia Fracchia, Piergiorgio Gentile

Research output: Contribution to journalReview article

Abstract

The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.
LanguageEnglish
JournalFrontiers in bioengineering and biotechnology
DOIs
Publication statusAccepted/In press - 6 Nov 2019

Fingerprint

Biomimetics
Honey
Anti-Bacterial Agents
Soft Tissue Infections
Gram-Positive Bacteria
Bacterial Infections
Emergencies
Endothelial Cells
Fibroblasts
Bacteria
Cell Line
Skin
Membranes
Pharmaceutical Preparations
Polyelectrolytes

Cite this

Mancuso, Elena ; Tonda-Turo, Chiara ; Ceresa, Chiara ; Pensabene, Virginia ; Connell, Simon ; Fracchia, Letizia ; Gentile, Piergiorgio. / Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties. In: Frontiers in bioengineering and biotechnology. 2019.
@article{e721651c70d54ae88392304dd82a1801,
title = "Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties",
abstract = "The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.",
author = "Elena Mancuso and Chiara Tonda-Turo and Chiara Ceresa and Virginia Pensabene and Simon Connell and Letizia Fracchia and Piergiorgio Gentile",
year = "2019",
month = "11",
day = "6",
doi = "10.3389/fbioe.2019.00344",
language = "English",
journal = "Frontiers in bioengineering and biotechnology",
issn = "2296-4185",

}

Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties. / Mancuso, Elena; Tonda-Turo, Chiara; Ceresa, Chiara; Pensabene, Virginia; Connell, Simon; Fracchia, Letizia; Gentile, Piergiorgio.

In: Frontiers in bioengineering and biotechnology, 06.11.2019.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties

AU - Mancuso, Elena

AU - Tonda-Turo, Chiara

AU - Ceresa, Chiara

AU - Pensabene, Virginia

AU - Connell, Simon

AU - Fracchia, Letizia

AU - Gentile, Piergiorgio

PY - 2019/11/6

Y1 - 2019/11/6

N2 - The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.

AB - The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.

U2 - 10.3389/fbioe.2019.00344

DO - 10.3389/fbioe.2019.00344

M3 - Review article

JO - Frontiers in bioengineering and biotechnology

T2 - Frontiers in bioengineering and biotechnology

JF - Frontiers in bioengineering and biotechnology

SN - 2296-4185

ER -