Potential anti-cancer effects of virgin olive oil phenols on colorectal carcinogenesis models in vitro

Chris Gill, A Boyd, E McDermott, M McCann, M Servili, R Selvaggini, A Taticchi, S Esposto, G Montedoro, H McGlynn, I Rowland

Research output: Contribution to journalArticle

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Abstract

The traditional Mediterranean diet is thought to represent a healthy lifestyle; especially given the incidence of several cancers including colorectal cancer is lower in Mediterranean countries compared to Northern Europe. Olive oil, a central component of the Mediterranean diet, is believed to beneficially affect numerous biological processes. We used phenols extracted from virgin olive oil on a series of in vitro systems that model important stages of colon carcinogenesis. The effect the extract on DNA damage induced by hydrogen peroxide was measured in HT29 cells using single cell microgel-electrophoresis. A significant anti-genotoxic linear trend (p = 0.011) was observed when HT29 cells were preincubated with olive oil phenols (0, 5, 10, 25, 50, 75, 100 mu g/ml) for 24 hr, then challenged with hydrogen peroxide. The olive oil phenols (50, 100 mu g/ml) significantly (p = 0.004, p = 0.002) improved barrier function of CACO2 cells after 48 hr as measured by transepithelial resistance. Significant inhibition of HT115 invasion (p < 0.01) was observed at olive oil phenols concentrations of 25, 50, 75, 100 mu g/ml using the matrigel invasion assay. No effect was observed on HT115 viability over the concentration range 0, 25, 50 75, 100 mu g/ml after 24 hr, although 75 and 100 mu g/ml olive oil phenols significantly inhibited HT115 cell attachment (p = 0.011, p = 0.006). Olive oil phenols had no significant effect on metastasis-related gene expression in HT115 cells. We have demonstrated that phenols extracted from virgin olive oil are capable of inhibiting several stages in colon carcinogenesis in vitro. (c) 2005 Wiley-Liss, Inc.
LanguageEnglish
Pages1-7
JournalInternational Journal of Cancer
Volume117
Issue number1
DOIs
Publication statusPublished - Oct 2005

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virgin olive oil
phenols
carcinogenesis
olive oil
Mediterranean diet
cells
colon
hydrogen peroxide
Northern European region
antineoplastic activity
colorectal neoplasms
Mediterranean region
metastasis
DNA damage
lifestyle
electrophoresis
viability
incidence
gene expression
neoplasms

Cite this

Gill, Chris ; Boyd, A ; McDermott, E ; McCann, M ; Servili, M ; Selvaggini, R ; Taticchi, A ; Esposto, S ; Montedoro, G ; McGlynn, H ; Rowland, I. / Potential anti-cancer effects of virgin olive oil phenols on colorectal carcinogenesis models in vitro. In: International Journal of Cancer. 2005 ; Vol. 117, No. 1. pp. 1-7.
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Gill, C, Boyd, A, McDermott, E, McCann, M, Servili, M, Selvaggini, R, Taticchi, A, Esposto, S, Montedoro, G, McGlynn, H & Rowland, I 2005, 'Potential anti-cancer effects of virgin olive oil phenols on colorectal carcinogenesis models in vitro', International Journal of Cancer, vol. 117, no. 1, pp. 1-7. https://doi.org/10.1002/ijc.21083

Potential anti-cancer effects of virgin olive oil phenols on colorectal carcinogenesis models in vitro. / Gill, Chris; Boyd, A; McDermott, E; McCann, M; Servili, M; Selvaggini, R; Taticchi, A; Esposto, S; Montedoro, G; McGlynn, H; Rowland, I.

In: International Journal of Cancer, Vol. 117, No. 1, 10.2005, p. 1-7.

Research output: Contribution to journalArticle

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