Porphyrin Production and Regulation in Cutaneous Propionibacteria

Emma Barnard, Tremylla Johnson, Tracy Ngo, Uma Arora, Gunilla Leuterio, Andrew Mc Dowell, Huiying Li

Research output: Contribution to journalArticle

Abstract

Porphyrins are intermediate metabolites in the biosynthesis of vital molecules, including heme, cobalamin, and chlorophyll. Bacterial porphyrins are known
to be proinflammatory, with high levels linked to inflammatory skin diseases. Propionibacterium species are dominant skin commensals and play essential roles in defending against pathogens and in triggering an inflammatory response. To better understand how the inflammatory potential of the skin microbiome may vary depending on its propionibacterial composition, we compared the production levels of porphyrins among Propionibacterium acnes, Propionibacterium granulosum, Propionibacterium avidum, and Propionibacterium humerusii strains. We found that porphyrin production varied among these species, with P. acnes type I strains producing significantly larger amounts of porphyrins than P. acnes type II and III strains and other Propionibacterium species. P. acnes strains that are highly associated with the common skin condition acne vulgaris responded to vitamin B12 supplementation with significantly higher porphyrin production. In contrast, vitamin B12 supplementation had no effect on the porphyrin production of health-associated P. acnes strains and other propionibacteria. We observed low-level porphyrin production in most Propionibacterium strains harboring the deoR repressor gene, with the exception of P. acnes strains belonging to type I clades IB-3 and IC. Our findings shed light on the proinflammatory potential of distinct phylogenetic lineages of P. acnes as well as other resident skin propionibacteria. We demonstrate that the overall species and strain composition is important in determining the metabolic output of the skin microbiome in health and disease.
LanguageEnglish
Article numbere00793-19
Pages1-10
Number of pages10
JournalmSphere
Volume5
Issue number1
Publication statusPublished - 15 Jan 2020

Fingerprint

Propionibacterium
Propionibacterium acnes
porphyrins
vitamin B12
Propionibacterium avidum
Propionibacterium granulosum
acne
skin diseases
heme
inflammation
biosynthesis
metabolites
chlorophyll
pathogens
phylogeny

Keywords

  • Propionibacterium acnes, propionibacteria, porphyrin, acne, skin microbiome

Cite this

Barnard, E., Johnson, T., Ngo, T., Arora, U., Leuterio, G., Mc Dowell, A., & Li, H. (2020). Porphyrin Production and Regulation in Cutaneous Propionibacteria. 5(1), 1-10. [e00793-19].
Barnard, Emma ; Johnson, Tremylla ; Ngo, Tracy ; Arora, Uma ; Leuterio, Gunilla ; Mc Dowell, Andrew ; Li, Huiying. / Porphyrin Production and Regulation in Cutaneous Propionibacteria. 2020 ; Vol. 5, No. 1. pp. 1-10.
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Barnard, E, Johnson, T, Ngo, T, Arora, U, Leuterio, G, Mc Dowell, A & Li, H 2020, 'Porphyrin Production and Regulation in Cutaneous Propionibacteria', vol. 5, no. 1, e00793-19, pp. 1-10.

Porphyrin Production and Regulation in Cutaneous Propionibacteria. / Barnard, Emma; Johnson, Tremylla; Ngo, Tracy; Arora, Uma; Leuterio, Gunilla; Mc Dowell, Andrew; Li, Huiying.

Vol. 5, No. 1, e00793-19, 15.01.2020, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Porphyrin Production and Regulation in Cutaneous Propionibacteria

AU - Barnard, Emma

AU - Johnson, Tremylla

AU - Ngo, Tracy

AU - Arora, Uma

AU - Leuterio, Gunilla

AU - Mc Dowell, Andrew

AU - Li, Huiying

PY - 2020/1/15

Y1 - 2020/1/15

N2 - Porphyrins are intermediate metabolites in the biosynthesis of vital molecules, including heme, cobalamin, and chlorophyll. Bacterial porphyrins are knownto be proinflammatory, with high levels linked to inflammatory skin diseases. Propionibacterium species are dominant skin commensals and play essential roles in defending against pathogens and in triggering an inflammatory response. To better understand how the inflammatory potential of the skin microbiome may vary depending on its propionibacterial composition, we compared the production levels of porphyrins among Propionibacterium acnes, Propionibacterium granulosum, Propionibacterium avidum, and Propionibacterium humerusii strains. We found that porphyrin production varied among these species, with P. acnes type I strains producing significantly larger amounts of porphyrins than P. acnes type II and III strains and other Propionibacterium species. P. acnes strains that are highly associated with the common skin condition acne vulgaris responded to vitamin B12 supplementation with significantly higher porphyrin production. In contrast, vitamin B12 supplementation had no effect on the porphyrin production of health-associated P. acnes strains and other propionibacteria. We observed low-level porphyrin production in most Propionibacterium strains harboring the deoR repressor gene, with the exception of P. acnes strains belonging to type I clades IB-3 and IC. Our findings shed light on the proinflammatory potential of distinct phylogenetic lineages of P. acnes as well as other resident skin propionibacteria. We demonstrate that the overall species and strain composition is important in determining the metabolic output of the skin microbiome in health and disease.

AB - Porphyrins are intermediate metabolites in the biosynthesis of vital molecules, including heme, cobalamin, and chlorophyll. Bacterial porphyrins are knownto be proinflammatory, with high levels linked to inflammatory skin diseases. Propionibacterium species are dominant skin commensals and play essential roles in defending against pathogens and in triggering an inflammatory response. To better understand how the inflammatory potential of the skin microbiome may vary depending on its propionibacterial composition, we compared the production levels of porphyrins among Propionibacterium acnes, Propionibacterium granulosum, Propionibacterium avidum, and Propionibacterium humerusii strains. We found that porphyrin production varied among these species, with P. acnes type I strains producing significantly larger amounts of porphyrins than P. acnes type II and III strains and other Propionibacterium species. P. acnes strains that are highly associated with the common skin condition acne vulgaris responded to vitamin B12 supplementation with significantly higher porphyrin production. In contrast, vitamin B12 supplementation had no effect on the porphyrin production of health-associated P. acnes strains and other propionibacteria. We observed low-level porphyrin production in most Propionibacterium strains harboring the deoR repressor gene, with the exception of P. acnes strains belonging to type I clades IB-3 and IC. Our findings shed light on the proinflammatory potential of distinct phylogenetic lineages of P. acnes as well as other resident skin propionibacteria. We demonstrate that the overall species and strain composition is important in determining the metabolic output of the skin microbiome in health and disease.

KW - Propionibacterium acnes, propionibacteria, porphyrin, acne, skin microbiome

M3 - Article

VL - 5

SP - 1

EP - 10

IS - 1

M1 - e00793-19

ER -

Barnard E, Johnson T, Ngo T, Arora U, Leuterio G, Mc Dowell A et al. Porphyrin Production and Regulation in Cutaneous Propionibacteria. 2020 Jan 15;5(1):1-10. e00793-19.