Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study

F Kee, C Morrison, AE Evans, E McCrum, D McMaster, J Dallongeville, V Nicaud, O Poirier, F Cambien

    Research output: Contribution to journalArticle

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    Abstract

    Background and objective-Studies in animal models and humans implicate cell adhesion molecules in atherogenesis but their role in mediating the risk of myocardial infarction is unclear. The ECTIM (etude cas-temoin Be l'infarctus myocarde) extension study was established to determine whether a previously implicated polymorphism of the P-selectin gene was associated with myocardial infarction risk in men and women in Belfast and Glasgow. Patients and study setting-696 cases with a recent myocardial infarction and 561 age matched controls (both male and female) were recruited into a case-control study in MONICA project areas of Belfast and Glasgow. Methods-Demographic and lifestyle information was collected by interview administered questionnaire, and each subject was examined and provided a blood sample for DNA extraction. The polymerase chain reaction (PCR) was used to amplify regions encompassing the P-selectin Thr-->Pro (A/C) polymorphism at position 715. Genotype odds ratios for myocardial infarction were estimated by logistic regression adjusted for population, age, and sex. Results-There was no significant association between conventional risk factors (such as hypercholesterolaemia, increased body mass index, or raised blood pressure) and either the rare or the common Pro(715) allele of the P-selectin gene in controls. Overall, comparing Pro(715)/Pro(715) and Pro(715)/Thr(715) With Thr(715)/Thr(715), With adjustment for centre, age, and sex, the odds ratio was 0.78 (95% confidence interval 0.60 to 1.00) (p = 0.054), indicating a ``protective'' effect of the less common Pro(715) allele. There was no significant heterogeneity in odds ratios between men and women either in this sample or when combined with the original ECTIM subjects. Conclusions-In a large population based study in two regions of the UK we have been able to corroborate the earlier ECTIM findings of a lower frequency of the Thr/Pro(715) polymorphism in subjects with myocardial infarction. An apparently ``protective effect'' of similar magnitude also seems to apply to women.
    LanguageEnglish
    Pages548-551
    JournalHeart
    Volume84
    Issue number5
    Publication statusPublished - Nov 2000

    Fingerprint

    P-Selectin
    Myocardial Infarction
    Genes
    Odds Ratio
    Alleles
    Sex Ratio
    Cell Adhesion Molecules
    Hypercholesterolemia
    Population
    Case-Control Studies
    Life Style
    Atherosclerosis
    Body Mass Index
    Animal Models
    Logistic Models
    Genotype
    Demography
    Confidence Intervals
    Interviews
    Blood Pressure

    Cite this

    Kee, F., Morrison, C., Evans, AE., McCrum, E., McMaster, D., Dallongeville, J., ... Cambien, F. (2000). Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study. Heart, 84(5), 548-551.
    Kee, F ; Morrison, C ; Evans, AE ; McCrum, E ; McMaster, D ; Dallongeville, J ; Nicaud, V ; Poirier, O ; Cambien, F. / Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study. In: Heart. 2000 ; Vol. 84, No. 5. pp. 548-551.
    @article{9e3cc49ebb844e8fafdaad7f2e70d022,
    title = "Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study",
    abstract = "Background and objective-Studies in animal models and humans implicate cell adhesion molecules in atherogenesis but their role in mediating the risk of myocardial infarction is unclear. The ECTIM (etude cas-temoin Be l'infarctus myocarde) extension study was established to determine whether a previously implicated polymorphism of the P-selectin gene was associated with myocardial infarction risk in men and women in Belfast and Glasgow. Patients and study setting-696 cases with a recent myocardial infarction and 561 age matched controls (both male and female) were recruited into a case-control study in MONICA project areas of Belfast and Glasgow. Methods-Demographic and lifestyle information was collected by interview administered questionnaire, and each subject was examined and provided a blood sample for DNA extraction. The polymerase chain reaction (PCR) was used to amplify regions encompassing the P-selectin Thr-->Pro (A/C) polymorphism at position 715. Genotype odds ratios for myocardial infarction were estimated by logistic regression adjusted for population, age, and sex. Results-There was no significant association between conventional risk factors (such as hypercholesterolaemia, increased body mass index, or raised blood pressure) and either the rare or the common Pro(715) allele of the P-selectin gene in controls. Overall, comparing Pro(715)/Pro(715) and Pro(715)/Thr(715) With Thr(715)/Thr(715), With adjustment for centre, age, and sex, the odds ratio was 0.78 (95{\%} confidence interval 0.60 to 1.00) (p = 0.054), indicating a ``protective'' effect of the less common Pro(715) allele. There was no significant heterogeneity in odds ratios between men and women either in this sample or when combined with the original ECTIM subjects. Conclusions-In a large population based study in two regions of the UK we have been able to corroborate the earlier ECTIM findings of a lower frequency of the Thr/Pro(715) polymorphism in subjects with myocardial infarction. An apparently ``protective effect'' of similar magnitude also seems to apply to women.",
    author = "F Kee and C Morrison and AE Evans and E McCrum and D McMaster and J Dallongeville and V Nicaud and O Poirier and F Cambien",
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    Kee, F, Morrison, C, Evans, AE, McCrum, E, McMaster, D, Dallongeville, J, Nicaud, V, Poirier, O & Cambien, F 2000, 'Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study', Heart, vol. 84, no. 5, pp. 548-551.

    Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study. / Kee, F; Morrison, C; Evans, AE; McCrum, E; McMaster, D; Dallongeville, J; Nicaud, V; Poirier, O; Cambien, F.

    In: Heart, Vol. 84, No. 5, 11.2000, p. 548-551.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study

    AU - Kee, F

    AU - Morrison, C

    AU - Evans, AE

    AU - McCrum, E

    AU - McMaster, D

    AU - Dallongeville, J

    AU - Nicaud, V

    AU - Poirier, O

    AU - Cambien, F

    PY - 2000/11

    Y1 - 2000/11

    N2 - Background and objective-Studies in animal models and humans implicate cell adhesion molecules in atherogenesis but their role in mediating the risk of myocardial infarction is unclear. The ECTIM (etude cas-temoin Be l'infarctus myocarde) extension study was established to determine whether a previously implicated polymorphism of the P-selectin gene was associated with myocardial infarction risk in men and women in Belfast and Glasgow. Patients and study setting-696 cases with a recent myocardial infarction and 561 age matched controls (both male and female) were recruited into a case-control study in MONICA project areas of Belfast and Glasgow. Methods-Demographic and lifestyle information was collected by interview administered questionnaire, and each subject was examined and provided a blood sample for DNA extraction. The polymerase chain reaction (PCR) was used to amplify regions encompassing the P-selectin Thr-->Pro (A/C) polymorphism at position 715. Genotype odds ratios for myocardial infarction were estimated by logistic regression adjusted for population, age, and sex. Results-There was no significant association between conventional risk factors (such as hypercholesterolaemia, increased body mass index, or raised blood pressure) and either the rare or the common Pro(715) allele of the P-selectin gene in controls. Overall, comparing Pro(715)/Pro(715) and Pro(715)/Thr(715) With Thr(715)/Thr(715), With adjustment for centre, age, and sex, the odds ratio was 0.78 (95% confidence interval 0.60 to 1.00) (p = 0.054), indicating a ``protective'' effect of the less common Pro(715) allele. There was no significant heterogeneity in odds ratios between men and women either in this sample or when combined with the original ECTIM subjects. Conclusions-In a large population based study in two regions of the UK we have been able to corroborate the earlier ECTIM findings of a lower frequency of the Thr/Pro(715) polymorphism in subjects with myocardial infarction. An apparently ``protective effect'' of similar magnitude also seems to apply to women.

    AB - Background and objective-Studies in animal models and humans implicate cell adhesion molecules in atherogenesis but their role in mediating the risk of myocardial infarction is unclear. The ECTIM (etude cas-temoin Be l'infarctus myocarde) extension study was established to determine whether a previously implicated polymorphism of the P-selectin gene was associated with myocardial infarction risk in men and women in Belfast and Glasgow. Patients and study setting-696 cases with a recent myocardial infarction and 561 age matched controls (both male and female) were recruited into a case-control study in MONICA project areas of Belfast and Glasgow. Methods-Demographic and lifestyle information was collected by interview administered questionnaire, and each subject was examined and provided a blood sample for DNA extraction. The polymerase chain reaction (PCR) was used to amplify regions encompassing the P-selectin Thr-->Pro (A/C) polymorphism at position 715. Genotype odds ratios for myocardial infarction were estimated by logistic regression adjusted for population, age, and sex. Results-There was no significant association between conventional risk factors (such as hypercholesterolaemia, increased body mass index, or raised blood pressure) and either the rare or the common Pro(715) allele of the P-selectin gene in controls. Overall, comparing Pro(715)/Pro(715) and Pro(715)/Thr(715) With Thr(715)/Thr(715), With adjustment for centre, age, and sex, the odds ratio was 0.78 (95% confidence interval 0.60 to 1.00) (p = 0.054), indicating a ``protective'' effect of the less common Pro(715) allele. There was no significant heterogeneity in odds ratios between men and women either in this sample or when combined with the original ECTIM subjects. Conclusions-In a large population based study in two regions of the UK we have been able to corroborate the earlier ECTIM findings of a lower frequency of the Thr/Pro(715) polymorphism in subjects with myocardial infarction. An apparently ``protective effect'' of similar magnitude also seems to apply to women.

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    Kee F, Morrison C, Evans AE, McCrum E, McMaster D, Dallongeville J et al. Polymorphisms of the P-selectin gene and risk of myocardial infarction in men and women in the ECTIM extension study. Heart. 2000 Nov;84(5):548-551.