Abstract
Von Willebrand’s disease (vWD) is the most common bleeding disorder, with approximately 1% of people affected globally [1]. It is characterised by a deficiency in the blood protein von Willebrand factor (vWF) [2]. The assessment of vWF in conjunction with platelet interaction is essential in understanding vWF activity in both healthy individuals and those with vWD. The fabrication of micro- and nanotopographies can facilitate blood-sensing technologies [3] within microfluidic flow chamber devices.
The most modern version of such devices, the dynamic platelet function assay (DPFA) facilitates dynamic blood flow at shear rates equivalent to arterial blood flow to better mimic the in vivo conditions [4]. However, the DPFA lacks specificity, due to the use of the endogenous vWF.
In this study, a range of polymer demixed surfaces previously studied [5] have been prepared from PMMA/PS and PMMA/PS + PS microsphere solutions under ambient conditions and incorporated within the flow chamber of the DPFA to investigate patient-specific protein and platelet binding. Furthermore, platelet kinetics have been investigated to determine any significant differences in platelet binding across substrate topographies.
The most modern version of such devices, the dynamic platelet function assay (DPFA) facilitates dynamic blood flow at shear rates equivalent to arterial blood flow to better mimic the in vivo conditions [4]. However, the DPFA lacks specificity, due to the use of the endogenous vWF.
In this study, a range of polymer demixed surfaces previously studied [5] have been prepared from PMMA/PS and PMMA/PS + PS microsphere solutions under ambient conditions and incorporated within the flow chamber of the DPFA to investigate patient-specific protein and platelet binding. Furthermore, platelet kinetics have been investigated to determine any significant differences in platelet binding across substrate topographies.
Original language | English |
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Pages | 46 |
Number of pages | 1 |
Publication status | Published online - 20 Jun 2023 |
Event | United Kingdom Society of Biomaterials Annual Conference: UKSB2023 - Ulster University, Belfast, United Kingdom Duration: 20 Jun 2023 → 21 Jun 2023 Conference number: 23 https://www.uksb.org.uk/wp-content/uploads/2023/06/Abstract-Book-Programme.pdf |
Conference
Conference | United Kingdom Society of Biomaterials Annual Conference |
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Country/Territory | United Kingdom |
City | Belfast |
Period | 20/06/23 → 21/06/23 |
Internet address |