Plasma induced DNA damage:: Comparison with the effects of ionizing radiation

Saša Lazović; Dejan Maletić; A Leskovac; J Filipović; Nevena  Puač; Gordana Malović; Gordana Joksić; Zoran Petrović

doi:10.1063/1.4896626

Plasma induced DNA damage: Comparison with the effects of ionizing radiation

Saša Lazović, Dejan Maletić, A Leskovac, J Filipović, Nevena Puač, Gordana Malović, Gordana Joksić, Zoran Petrović

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2 Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei.

Original language	English
Article number	124101
Number of pages	5
Journal	Applied Physics Letters
Volume	105
Issue number	12
DOIs	https://doi.org/10.1063/1.4896626
Publication status	Published (in print/issue) - 24 Sept 2014

Keywords

atmospheric pressure plasmas
DNA damage
apoptosis
single strand breaks
double strand breaks
gamma rays
gamma ray treatment

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10.1063/1.4896626

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title = "Plasma induced DNA damage:: Comparison with the effects of ionizing radiation",

abstract = "We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2 Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei.",

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T1 - Plasma induced DNA damage:

T2 - Comparison with the effects of ionizing radiation

AU - Lazović, Saša

AU - Maletić, Dejan

AU - Leskovac, A

AU - Filipović, J

AU - Puač, Nevena

AU - Malović, Gordana

AU - Joksić, Gordana

AU - Petrović, Zoran

PY - 2014/9/24

Y1 - 2014/9/24

N2 - We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2 Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei.

AB - We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2 Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei.

KW - atmospheric pressure plasmas

KW - DNA damage

KW - apoptosis

KW - single strand breaks

KW - double strand breaks

KW - gamma rays

KW - gamma ray treatment

UR - https://pure.ulster.ac.uk/en/publications/plasma-induced-dna-damage-comparison-with-the-effects-of-ionizing

U2 - 10.1063/1.4896626

DO - 10.1063/1.4896626

M3 - Article

SN - 0003-6951

VL - 105

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 12

M1 - 124101

ER -

Plasma induced DNA damage: Comparison with the effects of ionizing radiation

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Keywords

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