Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape

Nikolay A. Pchelintsev, Tony McBryan, Taranjit Singh Rai, John VanTuyn, Dominique Ray-Gallet, Geneviève Almouzni, Peter D. Adams

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and mechanism, we integrated HIRA, UBN1, ASF1a, and histone H3.3 chromatin immunoprecipitation sequencing and gene expression analyses. Most HIRA-binding sites colocalize with UBN1, ASF1a, and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and coregulator composition at different classes of HIRA-bound regulatory sites. Underscoring this, we report physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodeling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.

LanguageEnglish
Pages1012-1019
Number of pages8
JournalCell Reports
Volume3
Issue number4
DOIs
Publication statusPublished - 25 Apr 2013

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Histone Chaperones
Histones
Chromatin
Gene expression
Binding Sites
Gene Expression
Chromatin Assembly and Disassembly
Nucleosomes
Chromatin Immunoprecipitation
DNA Replication
Carrier Proteins
Transcription Factors
Adenosine Triphosphate
DNA
Chemical analysis

Cite this

Pchelintsev, N. A., McBryan, T., Rai, T. S., VanTuyn, J., Ray-Gallet, D., Almouzni, G., & Adams, P. D. (2013). Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape. Cell Reports, 3(4), 1012-1019. https://doi.org/10.1016/j.celrep.2013.03.026
Pchelintsev, Nikolay A. ; McBryan, Tony ; Rai, Taranjit Singh ; VanTuyn, John ; Ray-Gallet, Dominique ; Almouzni, Geneviève ; Adams, Peter D. / Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape. In: Cell Reports. 2013 ; Vol. 3, No. 4. pp. 1012-1019.
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Pchelintsev, NA, McBryan, T, Rai, TS, VanTuyn, J, Ray-Gallet, D, Almouzni, G & Adams, PD 2013, 'Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape', Cell Reports, vol. 3, no. 4, pp. 1012-1019. https://doi.org/10.1016/j.celrep.2013.03.026

Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape. / Pchelintsev, Nikolay A.; McBryan, Tony; Rai, Taranjit Singh; VanTuyn, John; Ray-Gallet, Dominique; Almouzni, Geneviève; Adams, Peter D.

In: Cell Reports, Vol. 3, No. 4, 25.04.2013, p. 1012-1019.

Research output: Contribution to journalArticle

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Pchelintsev NA, McBryan T, Rai TS, VanTuyn J, Ray-Gallet D, Almouzni G et al. Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape. Cell Reports. 2013 Apr 25;3(4):1012-1019. https://doi.org/10.1016/j.celrep.2013.03.026