TY - JOUR
T1 - Placing the HIRA Histone Chaperone Complex in the Chromatin Landscape
AU - Pchelintsev, Nikolay A.
AU - McBryan, Tony
AU - Rai, Taranjit Singh
AU - VanTuyn, John
AU - Ray-Gallet, Dominique
AU - Almouzni, Geneviève
AU - Adams, Peter D.
PY - 2013/4/25
Y1 - 2013/4/25
N2 - The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and mechanism, we integrated HIRA, UBN1, ASF1a, and histone H3.3 chromatin immunoprecipitation sequencing and gene expression analyses. Most HIRA-binding sites colocalize with UBN1, ASF1a, and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and coregulator composition at different classes of HIRA-bound regulatory sites. Underscoring this, we report physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodeling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.
AB - The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and mechanism, we integrated HIRA, UBN1, ASF1a, and histone H3.3 chromatin immunoprecipitation sequencing and gene expression analyses. Most HIRA-binding sites colocalize with UBN1, ASF1a, and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and coregulator composition at different classes of HIRA-bound regulatory sites. Underscoring this, we report physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodeling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.
UR - http://www.scopus.com/inward/record.url?scp=84876999181&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2013.03.026
DO - 10.1016/j.celrep.2013.03.026
M3 - Article
C2 - 23602572
AN - SCOPUS:84876999181
SN - 2211-1247
VL - 3
SP - 1012
EP - 1019
JO - Cell Reports
JF - Cell Reports
IS - 4
ER -
