Pioglitazone protects HDL2&3 against oxidation in overweight and obese men

Jane McEneny, Peter McPherson, Ian Young

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The worldwide epidemic of obesity is a major public health concern and is persuasively linked to the rising prevalence of diabetes and cardiovascular disease. Obesity is often associated with an abnormal lipoprotein profile, which may be partly negated by pioglitazone intervention, as this can influence the composition and oxidation characteristics of low-density lipoprotein (LDL). However, as pioglitazone's impact on these parameters within high-density lipoprotein (HDL), specifically HDL2&3, is absent from the literature, this study was performed to address this shortcoming.

Twenty men were randomized to placebo or pioglitazone (30 mg/day) for 12 weeks. HDL2&3 were isolated by rapidultracentrifugation. HDL2&3-cholesterol and phospholipid content were assessed by enzymatic assays and apolipoprotein AI (apoAI) content by single-radial immunodiffusion. HDL2&3 oxidation characteristics were assessed by monitoring conjugated diene production and paraoxonase-1 activity by spectrophotometric assays.

Compared with the placebo group, pioglitazone influenced the composition and oxidation potential of HDL2&3. Specifically, total cholesterol (P < 0.05), phospholipid (P < 0.001) and apoAI (P < 0.001) were enriched within HDL2. Furthermore, the resistance of HDL2&3 to oxidation (P < 0.05) and the activity of paroxonase-1 were also increased (P < 0.001).

Overall, these findings indicate that pioglitazone treatment induced antiatherogenic changes within HDL2&3, which may help reduce the incidence of premature cardiovascular disease linked with obesity.
Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalAnnals of Clinical Biochemistry
Issue number1
Early online date12 Nov 2012
Publication statusPublished (in print/issue) - Jan 2013


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