Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)

Richard Metcalfe, Francoise Koumanov, Jose Ruffino, Keith Stokes, Geoff Holman, Dylan Thomspon, Niels Vollaard

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Purpose: We have previously shown that six weeks of reduced-exertion high-intensity interval training (REHIT) improves V̇O2max in sedentary men and women, and insulin sensitivity in men. Here we present two studies examining the acute physiological and molecular responses to REHIT.Methods: In Study 1, five men and six women (age: 26±7 y, BMI: 23±3 kg∙m-2, V̇O2max: 51±11 ml∙kg-1∙min-1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30 and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21±2 y; 25±4 kg·m-2; 39±10 ml·kg-1·min-1) performed three trials (REHIT, 30 min cycling at 50% of V̇O2max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30 and 90 min post-exercise.Results: Acutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V̇O2, RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.Conclusions: Collectively these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise.
    LanguageEnglish
    JournalEuropean Journal of Applied Physiology
    Volume2015
    Early online date9 Jul 2015
    DOIs
    Publication statusE-pub ahead of print - 9 Jul 2015

    Fingerprint

    Exercise
    Appetite
    Glycogen
    Phosphorylation
    AMP-Activated Protein Kinases
    Ghrelin
    Plasma Volume
    Quadriceps Muscle
    p38 Mitogen-Activated Protein Kinases
    High-Intensity Interval Training
    Nonesterified Fatty Acids
    Cross-Over Studies
    Fatigue
    Insulin Resistance
    Lactic Acid
    Homeostasis
    Air
    Biopsy
    Gene Expression
    Glucose

    Keywords

    • HIT
    • Glycogen
    • Signalling pathways
    • AMPK
    • Exercise metabolism
    • Energy balance

    Cite this

    Metcalfe, Richard ; Koumanov, Francoise ; Ruffino, Jose ; Stokes, Keith ; Holman, Geoff ; Thomspon, Dylan ; Vollaard, Niels. / Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT). In: European Journal of Applied Physiology. 2015 ; Vol. 2015.
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    abstract = "Purpose: We have previously shown that six weeks of reduced-exertion high-intensity interval training (REHIT) improves V̇O2max in sedentary men and women, and insulin sensitivity in men. Here we present two studies examining the acute physiological and molecular responses to REHIT.Methods: In Study 1, five men and six women (age: 26±7 y, BMI: 23±3 kg∙m-2, V̇O2max: 51±11 ml∙kg-1∙min-1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30 and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21±2 y; 25±4 kg·m-2; 39±10 ml·kg-1·min-1) performed three trials (REHIT, 30 min cycling at 50{\%} of V̇O2max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30 and 90 min post-exercise.Results: Acutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V̇O2, RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.Conclusions: Collectively these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise.",
    keywords = "HIT, Glycogen, Signalling pathways, AMPK, Exercise metabolism, Energy balance",
    author = "Richard Metcalfe and Francoise Koumanov and Jose Ruffino and Keith Stokes and Geoff Holman and Dylan Thomspon and Niels Vollaard",
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    Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT). / Metcalfe, Richard; Koumanov, Francoise; Ruffino, Jose; Stokes, Keith; Holman, Geoff; Thomspon, Dylan; Vollaard, Niels.

    In: European Journal of Applied Physiology, Vol. 2015, 09.07.2015.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)

    AU - Metcalfe, Richard

    AU - Koumanov, Francoise

    AU - Ruffino, Jose

    AU - Stokes, Keith

    AU - Holman, Geoff

    AU - Thomspon, Dylan

    AU - Vollaard, Niels

    PY - 2015/7/9

    Y1 - 2015/7/9

    N2 - Purpose: We have previously shown that six weeks of reduced-exertion high-intensity interval training (REHIT) improves V̇O2max in sedentary men and women, and insulin sensitivity in men. Here we present two studies examining the acute physiological and molecular responses to REHIT.Methods: In Study 1, five men and six women (age: 26±7 y, BMI: 23±3 kg∙m-2, V̇O2max: 51±11 ml∙kg-1∙min-1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30 and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21±2 y; 25±4 kg·m-2; 39±10 ml·kg-1·min-1) performed three trials (REHIT, 30 min cycling at 50% of V̇O2max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30 and 90 min post-exercise.Results: Acutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V̇O2, RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.Conclusions: Collectively these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise.

    AB - Purpose: We have previously shown that six weeks of reduced-exertion high-intensity interval training (REHIT) improves V̇O2max in sedentary men and women, and insulin sensitivity in men. Here we present two studies examining the acute physiological and molecular responses to REHIT.Methods: In Study 1, five men and six women (age: 26±7 y, BMI: 23±3 kg∙m-2, V̇O2max: 51±11 ml∙kg-1∙min-1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30 and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21±2 y; 25±4 kg·m-2; 39±10 ml·kg-1·min-1) performed three trials (REHIT, 30 min cycling at 50% of V̇O2max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30 and 90 min post-exercise.Results: Acutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V̇O2, RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.Conclusions: Collectively these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise.

    KW - HIT

    KW - Glycogen

    KW - Signalling pathways

    KW - AMPK

    KW - Exercise metabolism

    KW - Energy balance

    U2 - 10.1007/s00421-015-3217-6

    DO - 10.1007/s00421-015-3217-6

    M3 - Article

    VL - 2015

    JO - European Journal of Applied Physiology

    T2 - European Journal of Applied Physiology

    JF - European Journal of Applied Physiology

    SN - 1439-6319

    ER -